catE

catE
168

catechol 2,3-dioxygenase, detoxification of environmental catechols, protects cells from intoxication by endogenous bacillibactin-derived catechol metabolites under iron-limited conditions

Locus
BSU_08240
Molecular weight
31.37 kDa
Isoelectric point
5.3
Protein length
285 aaSequenceBlast
Gene length
858 bpSequenceBlast
Function
detoxification of catechol
Product
catechol 2,3-dioxygenase
Essential
no
Synonyms
catE, yfiE
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG2514 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
898,010  898,867
Phenotypes of a mutant
not viable in the presence of catechol PubMed
The protein
Catalyzed reaction/ biological activity
catechol + O2 --> (2Z,4E)-2-hydroxy-6-oxohexa-2,4-dienoate + H+ (according to UniProt)
Protein family
extradiol ring-cleavage dioxygenase family (according to UniProt)
Domains
2 VOC domains (aa 9-126, aa 169-285) (according to UniProt)
Structure
P54721 (AlphaFold)
Expression and Regulation
Operons
Genes
catD-catE
Description
PubMed
Regulation
strong induction by diamide and quinones (such as catechol) (YodB, CatR) PubMed
induced by iron starvation (Fur) PubMed
Regulatory mechanism
YodB: repression, PubMed, in yodB regulon
CatR: repression, PubMed, in catR regulon
Fur: repression, PubMed, in fur regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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catD→catE

2025-04-16 04:58:40

Jstuelk

61

917958761b4b1f416996a2a063990c7a6fd008bc

2DEC0ECC579FFD7C687FEF51E93AD14225EB14B4

Biological materials
Mutant
MGNA-C295 (yfiE::erm), available at the NBRP B. subtilis, Japan
BKE08240 (catE::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GGTCATGCTGCATGCCTCTT,  downstream forward: _UP4_TAACTGCAATCCCCTTGCCG
BKK08240 (catE::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GGTCATGCTGCATGCCTCTT,  downstream forward: _UP4_TAACTGCAATCCCCTTGCCG
References
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AA75013C05E1A88FC51410F9F1B30EE7D15F34C2

Page visits: 4447

Time of last update: 2025-04-22 02:45:35

Author of last update: Melvin.boenninger