TuaD

• Description: UDP glucose 6-dehydrogenase
  
  

Gene name	tuaD
Synonyms	yvhD
Essential	no
Product	UDP glucose 6-dehydrogenase
Function	biosynthesis of teichuronic acid
MW, pI	49 kDa, 6.107
Gene length, protein length	1383 bp, 461 aa
Immediate neighbours	tuaE, tuaC
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU35580

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: UDP-glucose + 2 NAD⁺ + H₂O = UDP-glucuronate + 2 NADH (according to Swiss-Prot)

• Protein family: UDP-glucose/GDP-mannose dehydrogenase family (according to Swiss-Prot)

• Paralogous protein(s): YtcA, YwqF

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylation on a Tyr residue by PtkA PubMed

• Cofactor(s):

• Effectors of protein activity: PtkA-dependent phosphorylation stimulates TuaD activity PubMed

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure:

• Swiss prot entry: O32271

• KEGG entry: [3]

• E.C. number: 1.1.1.22

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:
  • expressed under conditions of phosphate limitation (PhoP) PubMed

• Regulatory mechanism:
  • PhoP: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

1. Mijakovic I, Poncet S, Boël G, Mazé A, Gillet S, Decottignies P, Grangeasse C, Jamet E, Doublet P, Le Maréchal P, Deutscher J (2003) Transmembrane modulator-dependent bacterial tyrosine kinase activates UDP-glucose dehydrogenases. EMBO J 22:4709-4718. PubMed
2. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed