Aag

• Description: general stress protein, similar to DNA-3-methyladenine glycosidase II
  
  

Gene name	aag
Synonyms	yxlJ
Essential	no
Product	unknown
Function	survival of salt and ethanol stresses
MW, pI	21 kDa, 6.117
Gene length, protein length	588 bp, 196 aa
Immediate neighbours	yxzF, katX
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU38620

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: removes hypoxanthine, 3-alkylated purines, and 1,N6-ethenoadenine from DNA; hypoxanthine and 1,N6-ethenoadenine are the preferred substrates PubMed

• Protein family: DNA glycosylase MPG family (according to Swiss-Prot) AAG family of 3-methyladenine DNA glycosylases

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• Swiss prot entry: P94378

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon:

• Sigma factor: SigB PubMed

• Regulation: induced by stress (SigB) PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Dirk Höper, Uwe Völker, Michael Hecker
Comprehensive characterization of the contribution of individual SigB-dependent general stress genes to stress resistance of Bacillus subtilis.
J Bacteriol: 2005, 187(8);2810-26
[PubMed:15805528] [WorldCat.org] [DOI] (P p)

Randi M Aamodt, Pål Ø Falnes, Rune F Johansen, Erling Seeberg, Magnar Bjørås
The Bacillus subtilis counterpart of the mammalian 3-methyladenine DNA glycosylase has hypoxanthine and 1,N6-ethenoadenine as preferred substrates.
J Biol Chem: 2004, 279(14);13601-6
[PubMed:14729667] [WorldCat.org] [DOI] (P p)

1. Höper et al. (2005) Comprehensive Characterization of the Contribution of Individual SigB-Dependent General Stress Genes to Stress Resistance of Bacillus subtilis. J. Bact. 187: 2810-2826 PubMed
2. Aamodt et al. (2004) The Bacillus subtilis counterpart of the mammalian 3-methyladenine DNA glycosylase has hypoxanthine and 1,N6-ethenoadenine as preferred substrates. J. Biol. Chem. 279: 13601-13606-page. PubMed