Difference between revisions of "SpoIIIAG"
(→Expression and regulation) |
|||
| Line 90: | Line 90: | ||
=Expression and regulation= | =Expression and regulation= | ||
| − | * '''Operon:''' ''[[spoIIIAA]]-[[spoIIIAB]]-[[spoIIIAC]]-[[spoIIIAD]]-[[spoIIIAE]]-[[spoIIIAF]]-[[spoIIIAG]]-[[spoIIIAH]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/18485064 PubMed] | + | * '''Operon:''' |
| + | ** ''[[spoIIIAA]]-[[spoIIIAB]]-[[spoIIIAC]]-[[spoIIIAD]]-[[spoIIIAE]]-[[spoIIIAF]]-[[spoIIIAG]]-[[spoIIIAH]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/18485064 PubMed] | ||
| + | ** ''[[spoIIIAG]]-[[spoIIIAH]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/17693505 PubMed] | ||
| − | |||
| − | * '''Regulation:''' expressed during sporulation in the mother cell ([[SigE]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/18485064 PubMed], switched off by [[SpoIIID]] [http://www.ncbi.nlm.nih.gov/sites/entrez/15383836 PubMed] | + | * '''[[Sigma factor]]:''' |
| + | ** both promoters: [[SigE]] [http://www.ncbi.nlm.nih.gov/sites/entrez/18485064 PubMed] | ||
| + | |||
| + | * '''Regulation:''' | ||
| + | ** both promoters: expressed during sporulation in the mother cell ([[SigE]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/18485064 PubMed], switched off by [[SpoIIID]] [http://www.ncbi.nlm.nih.gov/sites/entrez/15383836 PubMed] | ||
* '''Regulatory mechanism:''' [[SpoIIID]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/15383836 PubMed] | * '''Regulatory mechanism:''' [[SpoIIID]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/15383836 PubMed] | ||
| − | * '''Additional information:''' | + | * '''Additional information:''' the internal promoter is essential for sporulation, it is twice as active as the promoter in front of [[spoIIIAA]], suggesting that [[SpoIIIAG]] and [[SpoIIIAH]] are required in larger amounts as compared to the other products of the operon [http://www.ncbi.nlm.nih.gov/sites/entrez/17693505 PubMed] |
=Biological materials = | =Biological materials = | ||
Revision as of 09:42, 1 June 2009
- Description: required for SigG activation
| Gene name | spoIIIAG |
| Synonyms | |
| Essential | no |
| Product | unknown |
| Function | activation of SigG |
| MW, pI | 25 kDa, 6.407 |
| Gene length, protein length | 687 bp, 229 aa |
| Immediate neighbours | spoIIIAH, spoIIIAF |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Coordinates:
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization: membrane protein PubMed
Database entries
- Structure:
- Swiss prot entry: P49784
- KEGG entry: BSU24370
- E.C. number:
Additional information
Expression and regulation
- Operon:
- Regulation:
- Additional information: the internal promoter is essential for sporulation, it is twice as active as the promoter in front of spoIIIAA, suggesting that SpoIIIAG and SpoIIIAH are required in larger amounts as compared to the other products of the operon PubMed
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
- Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed
