Difference between revisions of "DivIVA"
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* '''Effectors of protein activity:''' | * '''Effectors of protein activity:''' | ||
| − | * '''Interactions:''' [[ | + | * '''Interactions:''' [[DivIVA]]-[[RacA]], [[DivIVA]]-[[MinJ]] |
| − | * '''Localization:''' | + | * '''Localization:''' DivIVA forms a ring at the site of cell division and is enriched at both cell poles. |
=== Database entries === | === Database entries === | ||
Revision as of 13:41, 29 May 2009
- Description: cell-division initiation protein (septum placement)
| Gene name | divIVA |
| Synonyms | ylmJ |
| Essential | no |
| Product | cell-division initiation protein |
| Function | septum placement |
| MW, pI | 19 kDa, 4.846 |
| Gene length, protein length | 492 bp, 164 aa |
| Immediate neighbours | ylmH, ileS |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
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Contents
The gene
Basic information
- Coordinates:
Phenotypes of a mutant
Deletion of divIVA leads to filamentation and polar divisions that in turn cause a minicell phenotype. A divIVA mutant has a severe sporulation defect.
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: DivIVA is required for polar localisation of MinCD via MinJ. It also recruits RacA to the distal pole of the prespore.
- Protein family: gpsB family (according to Swiss-Prot)
- Paralogous protein(s): GpsB
Extended information on the protein
- Kinetic information:
- Domains:
- Modification: The Mycobacterium DivIVA homologue Wag31 is phosphorylated at T73.
- Cofactor(s):
- Effectors of protein activity:
- Localization: DivIVA forms a ring at the site of cell division and is enriched at both cell poles.
Database entries
- Structure:
- Swiss prot entry: P71021
- KEGG entry: BSU15420
- E.C. number:
Additional information
Expression and regulation
- Operon: one gene cistron
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Leendert Hamoen, Centre for Bacterial Cell Biology, Newcastle upon Tyne, United Kingdom [3]
Imrich Barak, Slovak Academy of Science, Bratislava, Slovakia homepage
Your additional remarks
References
D H Edwards, J Errington
The Bacillus subtilis DivIVA protein targets to the division septum and controls the site specificity of cell division.
Mol Microbiol: 1997, 24(5);905-15
[PubMed:9219999]
[WorldCat.org]
[DOI]
(P p)
