Difference between revisions of "Sandbox"

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#REDIRECT [[bacA]]
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* '''Description:''' phosphotransferase of the sporulation initiation phosphorelay <br/><br/>
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{| align="right" border="1" cellpadding="2"
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|-
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|style="background:#ABCDEF;" align="center"|'''Gene name'''
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|''spo0F''
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|-
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|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
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|-
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|style="background:#ABCDEF;" align="center"| '''Essential''' || no
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|-
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|style="background:#ABCDEF;" align="center"| '''Product''' || phosphotransferase of the sporulation <br/>initiation phosphorelay
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|-
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|style="background:#ABCDEF;" align="center"|'''Function''' || initiation of sporulation
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|-
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 14 kDa, 4.697 
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|-
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|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 372 bp, 124 aa
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|-
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[fbaA]]'', ''[[ywjG]]''
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|-
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB15730&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|-
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|colspan="2" | '''Genetic context''' <br/> [[Image:spo0F_context.gif]]
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<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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|-
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|}
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__TOC__
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<br/><br/><br/>
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=The gene=
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=== Basic information ===
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* '''Coordinates:'''
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===Phenotypes of a mutant ===
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=== Database entries ===
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* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/spo0F.html]
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* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10411]
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=== Additional information===
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=The protein=
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 +
=== Basic information/ Evolution ===
 +
 
 +
* '''Catalyzed reaction/ biological activity:'''
 +
 
 +
* '''Protein family:'''
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* '''Paralogous protein(s):'''
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=== Extended information on the protein ===
 +
 
 +
* '''Kinetic information:'''
 +
 
 +
* '''Domains:'''
 +
 
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* '''Modification:'''
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* '''Cofactor(s):'''
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* '''Effectors of protein activity:'''
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* '''Interactions:''' [[Spo0F]]-[[Spo0E]],  [[RapA]]-[[Spo0F]],  [[Spo0F]]-[[RapB]],  [[Spo0F]]-[[KinA]],  [[Spo0F]]-[[Spo0B]]
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* '''Localization:''' cytoplasm (according to Swiss-Prot)
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=== Database entries ===
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* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1SRR 1SRR] (phosphatase resistant mutant),  [http://www.rcsb.org/pdb/explore.do?structureId=1F51 1F51] (complex with Spo0B),  [http://www.rcsb.org/pdb/explore.do?structureId=2JVK 2JVK] (Mutant L66A),  [http://www.rcsb.org/pdb/explore.do?structureId=2FSP 2FSP]
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* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P06628 P06628]
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* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU37130 BSU37130]
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* '''E.C. number:'''
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=== Additional information===
 +
 
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=Expression and regulation=
 +
 
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* '''Operon:'''
 +
 
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* '''[[Sigma factor]]:'''
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* '''Regulation:'''
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* '''Regulatory mechanism:'''
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* '''Additional information:'''
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=Biological materials =
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* '''Mutant:'''
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* '''Expression vector:'''
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* '''lacZ fusion:'''
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* '''GFP fusion:'''
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* '''two-hybrid system:'''
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 +
* '''Antibody:'''
 +
 
 +
=Labs working on this gene/protein=
 +
 
 +
=Your additional remarks=
 +
 
 +
=References=
 +
 
 +
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]

Revision as of 15:14, 25 May 2009

  • Description: phosphotransferase of the sporulation initiation phosphorelay

Gene name spo0F
Synonyms
Essential no
Product phosphotransferase of the sporulation
initiation phosphorelay
Function initiation of sporulation
MW, pI 14 kDa, 4.697
Gene length, protein length 372 bp, 124 aa
Immediate neighbours fbaA, ywjG
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
Spo0F context.gif
This image was kindly provided by SubtiList




The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization: cytoplasm (according to Swiss-Prot)

Database entries

  • Structure: 1SRR (phosphatase resistant mutant), 1F51 (complex with Spo0B), 2JVK (Mutant L66A), 2FSP
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed
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