Difference between revisions of "Sandbox"

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* '''Description:''' required for SigG activation <br/><br/>
+
#REDIRECT [[bacA]]
 
 
{| align="right" border="1" cellpadding="2"
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|''spoIIIAF''
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Product''' || unknown
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Function''' || activation of SigG
 
|-
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 23 kDa, 4.952 
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 618 bp, 206 aa
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[spoIIIAG]]'', ''[[spoIIIAE]]''
 
|-
 
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB14369&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
 
|-
 
|colspan="2" | '''Genetic context''' <br/> [[Image:spoIIIAF_context.gif]]
 
<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|}
 
 
 
__TOC__
 
 
 
<br/><br/>
 
 
 
=The gene=
 
 
 
=== Basic information ===
 
 
 
* '''Coordinates:'''
 
 
 
===Phenotypes of a mutant ===
 
 
 
=== Database entries ===
 
 
 
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/yqhV-spoIIIAABCDEFGH.html]
 
 
 
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11413]
 
 
 
=== Additional information===
 
 
 
 
 
=The protein=
 
 
 
=== Basic information/ Evolution ===
 
 
 
* '''Catalyzed reaction/ biological activity:'''
 
 
 
* '''Protein family:'''
 
 
 
* '''Paralogous protein(s):'''
 
 
 
=== Extended information on the protein ===
 
 
 
* '''Kinetic information:'''
 
 
 
* '''Domains:'''
 
 
 
* '''Modification:'''
 
 
 
* '''Cofactor(s):'''
 
 
 
* '''Effectors of protein activity:'''
 
 
 
* '''Interactions:'''
 
 
 
* '''Localization:''' membrane protein [http://www.ncbi.nlm.nih.gov/sites/entrez/18485064 PubMed]
 
 
 
=== Database entries ===
 
 
 
* '''Structure:'''
 
 
 
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P49783 P49783]
 
 
 
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU24380 BSU24380]
 
 
 
* '''E.C. number:'''
 
 
 
=== Additional information===
 
 
 
=Expression and regulation=
 
 
 
* '''Operon:''' ''[[spoIIIAA]]-[[spoIIIAB]]-[[spoIIIAC]]-[[spoIIIAD]]-[[spoIIIAE]]-[[spoIIIAF]]-[[spoIIIAG]]-[[spoIIIAH]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/18485064 PubMed]
 
 
 
* '''[[Sigma factor]]:''' [[SigE]] [http://www.ncbi.nlm.nih.gov/sites/entrez/18485064 PubMed]
 
 
 
* '''Regulation:''' expressed during sporulation in the mother cell ([[SigE]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/18485064 PubMed], switched off by [[SpoIIID]] [http://www.ncbi.nlm.nih.gov/sites/entrez/15383836 PubMed]
 
 
 
* '''Regulatory mechanism:''' [[SpoIIID]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/15383836 PubMed]
 
 
 
* '''Additional information:'''
 
 
 
=Biological materials =
 
 
 
* '''Mutant:'''
 
 
 
* '''Expression vector:'''
 
       
 
* '''lacZ fusion:'''
 
 
 
* '''GFP fusion:'''
 
 
 
* '''two-hybrid system:'''
 
 
 
* '''Antibody:'''
 
 
 
=Labs working on this gene/protein=
 
 
 
=Your additional remarks=
 
 
 
=References=
 
 
 
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]
 

Revision as of 15:02, 25 May 2009

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