Difference between revisions of "Sandbox"

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* '''Description:''' two-component sensor kinase, control of cellular responses to protein secretion stress <br/><br/>
+
* '''Description:''' serine protease Do (heat-shock protein) <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''cssS''
+
|''htrA''
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yvqB ''
+
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ykdA ''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || two-component sensor kinase (OmpR family)
+
|style="background:#ABCDEF;" align="center"| '''Product''' || serine protease Do (heat-shock protein)
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || control of cellular responses to protein secretion stress
+
|style="background:#ABCDEF;" align="center"|'''Function''' || probably involved in processing, maturation, or secretion of extracellular enzymes
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 51 kDa, 5.795  
+
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 47 kDa, 4.699  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1353 bp, 451 aa  
+
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1347 bp, 449 aa  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[cssR]]'', ''[[yuxN]]''
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ykcC]]'', ''[[proG]]''
 
|-
 
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB15292&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
+
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB13147&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
 
|-
 
|-
 
|-
 
|-
 
|-
 
|-
|colspan="2" | '''Genetic context''' <br/> [[Image:cssS_context.gif]]
+
|colspan="2" | '''Genetic context''' <br/> [[Image:htrA_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|-
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=== Database entries ===
 
=== Database entries ===
  
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/cssRS.html]
+
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/htrA.html]
  
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG14132]
+
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12608]
  
 
=== Additional information===
 
=== Additional information===
Line 56: Line 56:
 
* '''Catalyzed reaction/ biological activity:'''  
 
* '''Catalyzed reaction/ biological activity:'''  
  
* '''Protein family:'''
+
* '''Protein family:''' PDZ (DHR) domain (according to Swiss-Prot)
  
* '''Paralogous protein(s):'''[[YkoH]], [[KinE]], [[YclK]], [[YvrG]]
+
* '''Paralogous protein(s):''' [[YyxA]]
  
 
=== Extended information on the protein ===
 
=== Extended information on the protein ===
Line 64: Line 64:
 
* '''Kinetic information:'''
 
* '''Kinetic information:'''
  
* '''Domains:''' two transmembrane segments, C-terminal histidine phosphotransferase domain
+
* '''Domains:'''  
  
 
* '''Modification:'''
 
* '''Modification:'''
Line 74: Line 74:
 
* '''Interactions:'''
 
* '''Interactions:'''
  
* '''Localization:''' cell membrane (according to Swiss-Prot)
+
* '''Localization:''' cell membrane (according to Swiss-Prot),  extracellular (signal peptide) [http://www.ncbi.nlm.nih.gov/pubmed/18957862 PubMed]
  
 
=== Database entries ===
 
=== Database entries ===
Line 80: Line 80:
 
* '''Structure:'''
 
* '''Structure:'''
  
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/O32193 O32193]
+
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/O34358 O34358]
  
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU33020 BSU33020]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU12900 BSU12900]
  
 
* '''E.C. number:'''
 
* '''E.C. number:'''
Line 121: Line 121:
  
 
Test.
 
Test.
<pubmed> 18487328</pubmed>
+
<pubmed> 12850135</pubmed>
  
  
# Hyyryläinen HL, Bolhuis A, Darmon E, Muukkonen L, Koski P, Vitikainen M, Sarvas M, Prágai Z, Bron S, van Dijl JM, Kontinen VP. (2001) A novel two-component regulatory system in Bacillus subtilis for the survival of severe secretion stress. ''Mol Microbiol.'' '''Sep;41(5):''' 1159-72. [http://www.ncbi.nlm.nih.gov/sites/entrez/11555295 PubMed]
+
# Voigt et al. (2009) Cell physiology and protein secretion of ''Bacillus licheniformis'' compared to ''Bacillus subtilis''. ''J Mol Microbiol Biotechnol.'' '''16:''' 53-68 [http://www.ncbi.nlm.nih.gov/pubmed/18957862 PubMed]
 +
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]

Revision as of 02:11, 23 May 2009

  • Description: serine protease Do (heat-shock protein)

Gene name htrA
Synonyms ykdA
Essential no
Product serine protease Do (heat-shock protein)
Function probably involved in processing, maturation, or secretion of extracellular enzymes
MW, pI 47 kDa, 4.699
Gene length, protein length 1347 bp, 449 aa
Immediate neighbours ykcC, proG
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
HtrA context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: PDZ (DHR) domain (according to Swiss-Prot)
  • Paralogous protein(s): YyxA

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: cell membrane (according to Swiss-Prot), extracellular (signal peptide) PubMed

Database entries

  • Structure:
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Test.


  1. Voigt et al. (2009) Cell physiology and protein secretion of Bacillus licheniformis compared to Bacillus subtilis. J Mol Microbiol Biotechnol. 16: 53-68 PubMed
  2. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed