Difference between revisions of "Sandbox"

From SubtiWiki
Jump to: navigation, search
Line 1: Line 1:
* '''Description:''' similar to cell wall binding protein <br/><br/>
+
* '''Description:''' rRNA adenine dimethyltransferase <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''yabE''
+
|''ksgA''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
 
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
Line 10: Line 10:
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || unknown
+
|style="background:#ABCDEF;" align="center"| '''Product''' || rRNA adenine dimethyltransferase
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || unknown
+
|style="background:#ABCDEF;" align="center"|'''Function''' || resistance to kasugamycin
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 47 kDa, 9.282  
+
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 32 kDa, 5.712  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1311 bp, 437 aa  
+
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 876 bp, 292 aa  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yabD]]'', ''[[rnmV]]''
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[rnmV]]'', ''[[yabG]]''
 
|-
 
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB11816&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
+
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB11818&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
 
|-
 
|-
|colspan="2" | '''Genetic context''' <br/> [[Image:yabE_context.gif]]
+
|colspan="2" | '''Genetic context''' <br/> [[Image:ksgA_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|-
Line 41: Line 41:
 
=== Database entries ===
 
=== Database entries ===
  
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/yabE.html]
+
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/rnmV-ksgA.html]
  
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10103]
+
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10105]
  
 
=== Additional information===
 
=== Additional information===
Line 62: Line 62:
 
* '''Kinetic information:'''
 
* '''Kinetic information:'''
  
* '''Domains:''' contains a SPS domain
+
* '''Domains:'''  
  
 
* '''Modification:'''
 
* '''Modification:'''
Line 78: Line 78:
 
* '''Structure:'''
 
* '''Structure:'''
  
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P37546 P37546]
+
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P37468 P37468]
  
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU00400]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU00420]
  
 
* '''E.C. number:'''
 
* '''E.C. number:'''
Line 88: Line 88:
 
=Expression and regulation=
 
=Expression and regulation=
  
* '''Operon:''' ''yabE'' [http://www.ncbi.nlm.nih.gov/sites/entrez/19047346 PubMed]
+
* '''Operon:''' ''[[rnmV]]-[[ksgA]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/11233981 PubMed]  
  
* '''[[Sigma factor]]:''' [[SigA]] [http://www.ncbi.nlm.nih.gov/sites/entrez/19047346 PubMed]
+
* '''[[Sigma factor]]:'''  
  
 
* '''Regulation:'''  
 
* '''Regulation:'''  
Line 96: Line 96:
 
* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
  
* '''Additional information:''' expression is negatively controlled by a cis-acting antisense RNA that is dependent on [[SigX]] and [[SigM]] [http://www.ncbi.nlm.nih.gov/sites/entrez/19047346 PubMed]
+
* '''Additional information:'''
  
 
=Biological materials =
 
=Biological materials =
Line 113: Line 113:
  
 
=Labs working on this gene/protein=
 
=Labs working on this gene/protein=
[[John Helmann]], Cornell University, USA [http://www.micro.cornell.edu/research/labs/helmann-lab/index.cfm Homepage]
 
  
 
=Your additional remarks=
 
=Your additional remarks=
Line 119: Line 118:
 
=References=
 
=References=
  
# Eiamphungporn W, Helmann JD (2009) Extracytoplasmic function [[sigma factor]]s regulate expression of the ''Bacillus subtilis yabE'' gene via a cis-acting antisense RNA. ''J. Bacteriol.'' '''191:''' 1101-1105. [http://www.ncbi.nlm.nih.gov/sites/entrez/19047346 PubMed]
+
# Condon et al. (2001) Identification of the gene encoding the 5S ribosomal RNA maturase in ''Bacillus subtilis'': mature 5S rRNA is dispensable for ribosome function. ''RNA'' '''7:''' 242-253. [http://www.ncbi.nlm.nih.gov/sites/entrez/11233981 PubMed]
 +
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]

Revision as of 16:26, 4 May 2009

  • Description: rRNA adenine dimethyltransferase

Gene name ksgA
Synonyms
Essential no
Product rRNA adenine dimethyltransferase
Function resistance to kasugamycin
MW, pI 32 kDa, 5.712
Gene length, protein length 876 bp, 292 aa
Immediate neighbours rnmV, yabG
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
KsgA context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Condon et al. (2001) Identification of the gene encoding the 5S ribosomal RNA maturase in Bacillus subtilis: mature 5S rRNA is dispensable for ribosome function. RNA 7: 242-253. PubMed
  2. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed