Difference between revisions of "Sandbox"

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* '''Description:''' controls [[SigG]] activation<br/><br/>
+
* '''Description:''' hydrolysis of 5-bromo-4-chloroindolyl phosphate <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''csfB''
+
|''xpaC''
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yaaM ''
+
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Product''' ||  
+
|style="background:#ABCDEF;" align="center"| '''Product''' || unknown
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || control of [[SigG]] activation
+
|style="background:#ABCDEF;" align="center"|'''Function''' || unknown
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 7 kDa, 7.671  
+
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 23 kDa, 10.149  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 192 bp, 64 aa  
+
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 612 bp, 204 aa  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[rrnA-5S]]'', ''[[xpaC]]''
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[csfB]]'', ''[[yaaN]]''
 
|-
 
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB11800&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
+
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB11801&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
 
|-
 
|-
|colspan="2" | '''Genetic context''' <br/> [[Image:csfB_context.gif]]
+
|colspan="2" | '''Genetic context''' <br/> [[Image:xpaC_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|-
Line 39: Line 39:
 
===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
  
premature activation of [[SigG]] [http://www.ncbi.nlm.nih.gov/sites/entrez/17921305 PubMed]
 
 
=== Database entries ===
 
=== Database entries ===
  
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/csfB.html]
+
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/xpaC-yaaN.html]
  
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10088]
+
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10089]
  
 
=== Additional information===
 
=== Additional information===
Line 53: Line 52:
 
=== Basic information/ Evolution ===
 
=== Basic information/ Evolution ===
  
* '''Catalyzed reaction/ biological activity:''' prevents premature activation of SigG [http://www.ncbi.nlm.nih.gov/sites/entrez/17921305 PubMed]
+
* '''Catalyzed reaction/ biological activity:'''  
  
 
* '''Protein family:'''
 
* '''Protein family:'''
Line 79: Line 78:
 
* '''Structure:'''
 
* '''Structure:'''
  
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P37534 P37534]
+
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P37467 P37467]
  
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU00240]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU00250]
  
 
* '''E.C. number:'''
 
* '''E.C. number:'''
Line 89: Line 88:
 
=Expression and regulation=
 
=Expression and regulation=
  
* '''Operon:''' ''csfB''
+
* '''Operon:''' ''[[xpaC]]-[[yaaN]]''
  
* '''[[Sigma factor]]:''' [[SigF]] [http://www.ncbi.nlm.nih.gov/sites/entrez/16497325 PubMed]
+
* '''[[Sigma factor]]:''' [[SigW]] [http://www.ncbi.nlm.nih.gov/sites/entrez/9987136 PubMed]
  
* '''Regulation:'''  
+
* '''Regulation:''' expressed in stationary phase
  
 
* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
Line 119: Line 118:
 
=References=
 
=References=
  
# Wang et al. (2006) The forespore line of gene expression in ''Bacillus subtilis''. ''J. Mol. Biol.'' '''358:''' 16-37. [http://www.ncbi.nlm.nih.gov/sites/entrez/16497325 PubMed]
+
# Huang et al. (1999) Identification of target promoters for the Bacillus subtilis extracytoplasmic function [[sigma factor]], sigma W. ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]
# Chary et al. (2007) Expression of the sigmaF-directed ''csfB'' locus prevents premature appearance of sigmaG activity during sporulation of ''Bacillus subtilis''.''J. Bacteriol.'' '''189:''' 8754-8757. [http://www.ncbi.nlm.nih.gov/sites/entrez/17921305 PubMed]
+
# Author1, Author2 & Author3 (year) Title ''Mol. Microbiol.'' '''31:''' 361-371. [http://www.ncbi.nlm.nih.gov/sites/entrez/9987136 PubMed]
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]
 

Revision as of 16:25, 4 May 2009

  • Description: hydrolysis of 5-bromo-4-chloroindolyl phosphate

Gene name xpaC
Synonyms
Essential no
Product unknown
Function unknown
MW, pI 23 kDa, 10.149
Gene length, protein length 612 bp, 204 aa
Immediate neighbours csfB, yaaN
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
XpaC context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation: expressed in stationary phase
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Huang et al. (1999) Identification of target promoters for the Bacillus subtilis extracytoplasmic function sigma factor, sigma W. Journal volume: page-page. PubMed
  2. Author1, Author2 & Author3 (year) Title Mol. Microbiol. 31: 361-371. PubMed