Difference between revisions of "Sandbox"
| Line 1: | Line 1: | ||
| − | * '''Description:''' | + | * '''Description:''' general stress protein, survival of ethanol stress, spore coat protein <br/><br/> |
{| align="right" border="1" cellpadding="2" | {| align="right" border="1" cellpadding="2" | ||
|- | |- | ||
|style="background:#ABCDEF;" align="center"|'''Gene name''' | |style="background:#ABCDEF;" align="center"|'''Gene name''' | ||
| − | |'' | + | |''yaaH'' |
|- | |- | ||
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' '' | |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' '' | ||
|- | |- | ||
| − | |style="background:#ABCDEF;" align="center"| '''Essential''' || | + | |style="background:#ABCDEF;" align="center"| '''Essential''' || no |
|- | |- | ||
| − | |style="background:#ABCDEF;" align="center"| '''Product''' || | + | |style="background:#ABCDEF;" align="center"| '''Product''' || spore coat protein |
|- | |- | ||
| − | |style="background:#ABCDEF;" align="center"|'''Function''' || | + | |style="background:#ABCDEF;" align="center"|'''Function''' || survival of ethanol stress |
|- | |- | ||
| − | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 48 kDa, 5. | + | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 48 kDa, 5.618 |
|- | |- | ||
| − | |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || | + | |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1281 bp, 427 aa |
|- | |- | ||
| − | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ | + | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[dgk]]'', ''[[yaaI]]'' |
|- | |- | ||
| − | |colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS: | + | |colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB11792]+-newId sequences] <br/> (Barbe ''et al.'', 2009)''' |
|- | |- | ||
| − | |colspan="2" | '''Genetic context''' <br/> [[Image: | + | |colspan="2" | '''Genetic context''' <br/> [[Image:yaaH_context.gif]] |
<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div> | <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div> | ||
|- | |- | ||
| Line 38: | Line 38: | ||
===Phenotypes of a mutant === | ===Phenotypes of a mutant === | ||
| − | |||
| − | |||
=== Database entries === | === Database entries === | ||
| − | * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ | + | * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/yaaH.html] |
| − | * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+ | + | * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10080] |
=== Additional information=== | === Additional information=== | ||
| Line 74: | Line 72: | ||
* '''Interactions:''' | * '''Interactions:''' | ||
| − | * '''Localization:''' | + | * '''Localization:''' spore wall (according to Swiss-Prot), spore coat [http://www.ncbi.nlm.nih.gov/sites/entrez/11964120 PubMed] |
=== Database entries === | === Database entries === | ||
| Line 80: | Line 78: | ||
* '''Structure:''' | * '''Structure:''' | ||
| − | * '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/ | + | * '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P37531 P37531] |
| − | * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+ | + | * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU00160] |
* '''E.C. number:''' | * '''E.C. number:''' | ||
=== Additional information=== | === Additional information=== | ||
| + | |||
| + | required for survival of ethanol stress [http://www.ncbi.nlm.nih.gov/sites/entrez/15805528 PubMed] | ||
=Expression and regulation= | =Expression and regulation= | ||
| − | * '''Operon:''' '' | + | * '''Operon:''' ''yaaH'' [http://www.ncbi.nlm.nih.gov/sites/entrez/10419957 PubMed] |
| − | * '''[[Sigma factor]]:''' | + | * '''[[Sigma factor]]:''' [[SigE]] [http://www.ncbi.nlm.nih.gov/sites/entrez/10419957 PubMed], [[SigB]] [http://www.ncbi.nlm.nih.gov/sites/entrez/15805528 PubMed] |
| − | * '''Regulation:''' induced | + | * '''Regulation:''' induced by stress ([[SigB]]) [http://www.ncbi.nlm.nih.gov/pubmed/15805528 PubMed], expressed during sporulation [http://www.ncbi.nlm.nih.gov/sites/entrez/10419957 PubMed] |
| − | * '''Regulatory mechanism:''' | + | * '''Regulatory mechanism:''' |
* '''Additional information:''' | * '''Additional information:''' | ||
| Line 120: | Line 120: | ||
=References= | =References= | ||
| − | # | + | # Höper et al. (2005) Comprehensive Characterization of the Contribution of Individual [[SigB]]-Dependent General Stress Genes to Stress Resistance of ''Bacillus subtilis''. J. Bact. '''187:''' 2810-2826 [http://www.ncbi.nlm.nih.gov/pubmed/15805528 PubMed] |
| − | '' | + | # Kodama et al. (1999) The ''Bacillus subtilis yaaH'' gene is transcribed by SigE RNA polymerase during sporulation, and its product is involved in germination of spores.''J. Bacteriol.'' '''181:''' 4584-4591. [http://www.ncbi.nlm.nih.gov/sites/entrez/10419957 PubMed] |
| + | # Höper et al. (2005) Comprehensive characterization of the contribution of individual SigB-dependent general stress genes to stress resistance of ''Bacillus subtilis''.''J.Bacteriol.'' '''187:''' 2810-2826. [http://www.ncbi.nlm.nih.gov/sites/entrez/15805528 PubMed] | ||
| + | # Aronson (2002) Sporulation and delta-endotoxin synthesis by ''Bacillus thuringiensis''.''Cell. Mol. Life Sci.'' '''59:''' 417-425. [http://www.ncbi.nlm.nih.gov/sites/entrez/11964120 PubMed] | ||
Revision as of 16:24, 4 May 2009
- Description: general stress protein, survival of ethanol stress, spore coat protein
| Gene name | yaaH |
| Synonyms | |
| Essential | no |
| Product | spore coat protein |
| Function | survival of ethanol stress |
| MW, pI | 48 kDa, 5.618 |
| Gene length, protein length | 1281 bp, 427 aa |
| Immediate neighbours | dgk, yaaI |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Coordinates:
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization: spore wall (according to Swiss-Prot), spore coat PubMed
Database entries
- Structure:
- Swiss prot entry: P37531
- KEGG entry: [3]
- E.C. number:
Additional information
required for survival of ethanol stress PubMed
Expression and regulation
- Operon: yaaH PubMed
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
- Höper et al. (2005) Comprehensive Characterization of the Contribution of Individual SigB-Dependent General Stress Genes to Stress Resistance of Bacillus subtilis. J. Bact. 187: 2810-2826 PubMed
- Kodama et al. (1999) The Bacillus subtilis yaaH gene is transcribed by SigE RNA polymerase during sporulation, and its product is involved in germination of spores.J. Bacteriol. 181: 4584-4591. PubMed
- Höper et al. (2005) Comprehensive characterization of the contribution of individual SigB-dependent general stress genes to stress resistance of Bacillus subtilis.J.Bacteriol. 187: 2810-2826. PubMed
- Aronson (2002) Sporulation and delta-endotoxin synthesis by Bacillus thuringiensis.Cell. Mol. Life Sci. 59: 417-425. PubMed
