Difference between revisions of "Sandbox"

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* '''Description:''' stimulation of Spx degradation <br/><br/>
+
* '''Description:''' hydroxyethylthiazole phosphate biosynthesis <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''yjbH''
+
|''thiS''
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
+
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yjbS ''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || unknown
+
|style="background:#ABCDEF;" align="center"| '''Product''' || unknown  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || stimulation of Spx degradation
+
|style="background:#ABCDEF;" align="center"|'''Function''' || biosynthesis of thiamine
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 31 kDa, 5.206  
+
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 7 kDa, 5.501  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 825 bp, 275 aa  
+
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 198 bp, 66 aa  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[pepF]]'', ''[[yjbI]]''
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[goxB]]'', ''[[thiG]]''
 
|-
 
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB13012&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
+
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB13025&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
 
|-
 
|-
|colspan="2" | '''Genetic context''' <br/> [[Image:yjbH_context.gif]]
+
|colspan="2" | '''Genetic context''' <br/> [[Image:thiS_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|-
Line 41: Line 41:
 
=== Database entries ===
 
=== Database entries ===
  
* '''DBTBS entry:''' no entry
+
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/tenAI-goxB-thiSGF-yjbV.html]
  
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13137]
+
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13148]
  
 
=== Additional information===
 
=== Additional information===
Line 52: Line 52:
 
=== Basic information/ Evolution ===
 
=== Basic information/ Evolution ===
  
* '''Catalyzed reaction/ biological activity:''' adaptor protein for ClpXP-catalyzed Spx degradation [http://www.ncbi.nlm.nih.gov/sites/entrez/19074380 PubMed]
+
* '''Catalyzed reaction/ biological activity:'''  
  
 
* '''Protein family:'''
 
* '''Protein family:'''
Line 66: Line 66:
 
* '''Modification:'''
 
* '''Modification:'''
  
* '''Cofactor(s):'''contains Zn atoms (coordinated by the N-terminal His-rich region) [http://www.ncbi.nlm.nih.gov/sites/entrez/19074380 PubMed]
+
* '''Cofactor(s):'''
  
* '''Effectors of protein activity:''' Zn atom is released upon treatment with strong oxidants [http://www.ncbi.nlm.nih.gov/sites/entrez/19074380 PubMed]
+
* '''Effectors of protein activity:'''
  
* '''Interactions:''' [[ClpXP]],  YjbH-[[Spx]] [http://www.ncbi.nlm.nih.gov/sites/entrez/19074380 PubMed]
+
* '''Interactions:''' [[ThiS]]-[[ThiG]],
  
 
* '''Localization:'''
 
* '''Localization:'''
Line 80: Line 80:
 
* '''Swiss prot entry:'''
 
* '''Swiss prot entry:'''
  
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU11550]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU11680]
  
 
* '''E.C. number:'''
 
* '''E.C. number:'''
Line 113: Line 113:
  
 
=Labs working on this gene/protein=
 
=Labs working on this gene/protein=
 
[[Peter Zuber]], Oregon Health and Science University, USA
 
[http://www.ogi.edu/people/dsp_person.cfm?person_id=411D6801-2A56-D16D-58A06B4480EDB9C7 Homepage]
 
 
[[Claes von Wachenfeldt]], Lund University, Sweden [http://aron.ldc.lu.se/kundwebb/cellorg/mibiol/research/wachen/index.htm Homepage]
 
  
 
=Your additional remarks=
 
=Your additional remarks=
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=References=
 
=References=
  
# Garg et al. 2009. The YjbH protein of Bacillus subtilis enhances ClpXP-catalyzed proteolysis of Spx. J. Bacteriol. 191: 1268-1277. [http://www.ncbi.nlm.nih.gov/sites/entrez/19074380 PubMed]
+
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]
# Larsson, J. T., A. Rogstam, and C. von Wachenfeldt. 2007. YjbH is a novel negative effector of the disulphide stress regulator, Spx, in Bacillus subtilis. Mol. Microbiol. 66:669-684. [http://www.ncbi.nlm.nih.gov/sites/entrez/17908206 PubMed]
 

Revision as of 23:55, 27 April 2009

  • Description: hydroxyethylthiazole phosphate biosynthesis

Gene name thiS
Synonyms yjbS
Essential no
Product unknown
Function biosynthesis of thiamine
MW, pI 7 kDa, 5.501
Gene length, protein length 198 bp, 66 aa
Immediate neighbours goxB, thiG
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
ThiS context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization:

Database entries

  • Structure:
  • Swiss prot entry:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed