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| − | * '''Description:''' general stress protein, similar to L25 <br/><br/>
| + | #REDIRECT [[SubA]] |
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| − | {| align="right" border="1" cellpadding="2"
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| − | |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| − | |''ctc''
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| − | |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
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| − | |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| − | |style="background:#ABCDEF;" align="center"| '''Product''' || ribosomal protein
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| − | |style="background:#ABCDEF;" align="center"|'''Function''' || unknown
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| − | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 21 kDa, 4.216
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| − | |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 612 bp, 204 aa
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| − | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[prs]]'', ''[[spoVC]]''
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| − | |colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB11828]+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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| − | |colspan="2" | '''Genetic context''' <br/> [[Image:ctc_context.gif]]
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| − | <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| − | |}
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| − | __TOC__
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| − | <br/><br/>
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| − | =The gene=
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| − | === Basic information ===
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| − | * '''Coordinates:'''
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| − | ===Phenotypes of a mutant ===
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| − | === Database entries ===
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| − | * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/gcaD-prs-ctc.html]
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| − | * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10115]
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| − | === Additional information===
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| − | =The protein=
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| − | === Basic information/ Evolution ===
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| − | * '''Catalyzed reaction/ biological activity:'''
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| − | * '''Protein family:''' Ctc subfamily
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| − | * '''Paralogous protein(s):'''
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| − | | |
| − | === Extended information on the protein ===
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| − | * '''Kinetic information:'''
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| − | * '''Domains:'''
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| − | * '''Modification:'''
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| − | * '''Cofactor(s):'''
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| − | * '''Effectors of protein activity:'''
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| − | * '''Interactions:''' binds 5S rRNA [http://www.ncbi.nlm.nih.gov/sites/entrez/12432960 PubMed]
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| − | * '''Localization:''' in the ribosome (large subunit) [http://www.ncbi.nlm.nih.gov/sites/entrez/12432960 PubMed]
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| − | | |
| − | === Database entries ===
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| − | * '''Structure:'''
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| − | * '''Swiss prot entry:'''
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| − | * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU00520]
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| − | * '''E.C. number:'''
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| − | === Additional information===
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| − | :* subject to Clp-dependent proteolysis upon glucose starvation [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=+17981983 PubMed]
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| − | =Expression and regulation=
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| − | * '''Operon:''' ''[[gcaD]]-[[prs]]-[[ctc]]'', ''[[ctc]]''
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| − | * '''[[Sigma factor]]:''' ''[[gcaD]]'': [[SigA]], ''[[ctc]]'': [[SigB]]
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| − | * '''Regulation:''' induced by stress ([[SigB]]) [http://www.ncbi.nlm.nih.gov/pubmed/15805528 PubMed], induced under stress conditions
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| − | * '''Regulatory mechanism:''' alternative [[sigma factor]], [[SigB]]
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| − | * '''Additional information:''' subject to Clp-dependent proteolysis upon glucose starvation [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=+17981983 PubMed]
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| − | =Biological materials =
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| − | * '''Mutant:'''
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| − | * '''Expression vector:'''
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| − | * '''lacZ fusion:'''
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| − | * '''GFP fusion:'''
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| − | * '''two-hybrid system:'''
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| − | * '''Antibody:''' available in [[Stülke]] lab
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| − | =Labs working on this gene/protein=
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| − | =Your additional remarks=
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| − | =References=
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| − | # Höper et al. (2005) Comprehensive Characterization of the Contribution of Individual [[SigB]]-Dependent General Stress Genes to Stress Resistance of ''Bacillus subtilis''. J. Bact. '''187:''' 2810-2826 [http://www.ncbi.nlm.nih.gov/pubmed/15805528 PubMed]
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| − | # Gerth et al. (2008) Clp-dependent proteolysis down-regulates central metabolic pathways in glucose-starved Bacillus subtilis. J Bacteriol 190:321-331 [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=+17981983 PubMed]
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| − | # Schmalisch, M., Langbein, I. & Stülke, J. (2002) The general stress protein Ctc of ''Bacillus subtilis'' is a ribosomal protein. J. Mol. Microbiol. Biotechnol. 4: 495-501. [http://www.ncbi.nlm.nih.gov/sites/entrez/12432960 PubMed]
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| − | # Ollington, J. F., Haldenwang, W. G., Huynh, T. V. and Losick, R. 1981. Developmentally regulated transcription in a cloned segment of the ''Bacillus subtilis'' chromosome. J. Bacteriol. 147: 432-442. [http://www.ncbi.nlm.nih.gov/sites/entrez/6790515 PubMed]
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| − | # Truitt, C. L., Weaver, E. A. and Haldenwang, W. G. 1988. Effects on growth and sporulation of inactivation of a ''Bacillus subtilis'' gene (''ctc'') transcribed in vitro by minor vegetative cell RNA polymerases (E-sigma(37), E-sigma(32)). Mol. Gen. Genet. 212: 166-171. [http://www.ncbi.nlm.nih.gov/sites/entrez/2836704 PubMed]
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| − | # Haldenwang, W. G. & Losick, R. (1979). A modified RNA polymerase transcribes a cloned gene under sporulation control in ''Bacillus subtilis''. Nature 282, 256-260 [http://www.ncbi.nlm.nih.gov/pubmed/116131 PubMed]
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| − | # Gongadze GM, Korepanov AP, Korobeinikova AV, Garber MB (2008) Bacterial 5S rRNA-binding proteins of the CTC family. ''Biochemistry (Moscow)'' '''73:''' 1405-1417. [http://www.ncbi.nlm.nih.gov/sites/entrez/19216708 PubMed]
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| − | # Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]
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