Difference between revisions of "Sandbox"

From SubtiWiki
Jump to: navigation, search
(Blanked the page)
Line 1: Line 1:
 +
* '''Description:''' rRNA adenine dimethyltransferase <br/><br/>
  
 +
{| align="right" border="1" cellpadding="2"
 +
|-
 +
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 +
|''ksgA''
 +
|-
 +
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
 +
|-
 +
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
 +
|-
 +
|style="background:#ABCDEF;" align="center"| '''Product''' || rRNA adenine dimethyltransferase
 +
|-
 +
|style="background:#ABCDEF;" align="center"|'''Function''' || resistance to kasugamycin
 +
|-
 +
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 32 kDa, 5.712 
 +
|-
 +
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 876 bp, 292 aa
 +
|-
 +
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[rnmV]]'', ''[[yabG]]''
 +
|-
 +
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB11818&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
 +
|-
 +
|colspan="2" | '''Genetic context''' <br/> [[Image:ksgA_context.gif]]
 +
<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 +
|-
 +
|}
 +
 +
__TOC__
 +
 +
<br/><br/>
 +
 +
=The gene=
 +
 +
=== Basic information ===
 +
 +
* '''Coordinates:'''
 +
 +
===Phenotypes of a mutant ===
 +
 +
=== Database entries ===
 +
 +
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/rnmV-ksgA.html]
 +
 +
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10105]
 +
 +
=== Additional information===
 +
 +
 +
=The protein=
 +
 +
=== Basic information/ Evolution ===
 +
 +
* '''Catalyzed reaction/ biological activity:'''
 +
 +
* '''Protein family:''' KsgA subfamily
 +
 +
* '''Paralogous protein(s):'''
 +
 +
=== Extended information on the protein ===
 +
 +
* '''Kinetic information:'''
 +
 +
* '''Domains:'''
 +
 +
* '''Modification:'''
 +
 +
* '''Cofactor(s):'''
 +
 +
* '''Effectors of protein activity:'''
 +
 +
* '''Interactions:'''
 +
 +
* '''Localization:'''
 +
 +
=== Database entries ===
 +
 +
* '''Structure:'''
 +
 +
* '''Swiss prot entry:'''
 +
 +
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU00420]
 +
 +
* '''E.C. number:'''
 +
 +
=== Additional information===
 +
 +
=Expression and regulation=
 +
 +
* '''Operon:''' ''[[rnmV]]-[[ksgA]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/11233981 PubMed]
 +
 +
* '''[[Sigma factor]]:'''
 +
 +
* '''Regulation:'''
 +
 +
* '''Regulatory mechanism:'''
 +
 +
* '''Additional information:'''
 +
 +
=Biological materials =
 +
 +
* '''Mutant:'''
 +
 +
* '''Expression vector:'''
 +
       
 +
* '''lacZ fusion:'''
 +
 +
* '''GFP fusion:'''
 +
 +
* '''two-hybrid system:'''
 +
 +
* '''Antibody:'''
 +
 +
=Labs working on this gene/protein=
 +
 +
=Your additional remarks=
 +
 +
=References=
 +
 +
# Condon et al. (2001) Identification of the gene encoding the 5S ribosomal RNA maturase in ''Bacillus subtilis'': mature 5S rRNA is dispensable for ribosome function. ''RNA'' '''7:''' 242-253. [http://www.ncbi.nlm.nih.gov/sites/entrez/11233981 PubMed]
 +
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]

Revision as of 17:25, 21 April 2009

  • Description: rRNA adenine dimethyltransferase

Gene name ksgA
Synonyms
Essential no
Product rRNA adenine dimethyltransferase
Function resistance to kasugamycin
MW, pI 32 kDa, 5.712
Gene length, protein length 876 bp, 292 aa
Immediate neighbours rnmV, yabG
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
KsgA context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: KsgA subfamily
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • Swiss prot entry:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Condon et al. (2001) Identification of the gene encoding the 5S ribosomal RNA maturase in Bacillus subtilis: mature 5S rRNA is dispensable for ribosome function. RNA 7: 242-253. PubMed
  2. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed