Difference between revisions of "Sandbox"

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* '''Description:''' secondary transporter of divalent metal ions/citrate complexes <br/><br/>
+
* '''Description:''' catalase, general stress protein <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''citH''
+
|''katE''
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yxiQ ''
+
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''katB ''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || secondary transporter of divalent metal ions/citrate complexes
+
|style="background:#ABCDEF;" align="center"| '''Product''' || catalase 2
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || citrate  uptake
+
|style="background:#ABCDEF;" align="center"|'''Function''' || detoxification (degradation) of hydrogen peroxide
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 45 kDa, 9.278  
+
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 77 kDa, 5.845  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1278 bp, 426 aa  
+
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 2058 bp, 686 aa  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[katE]]'', ''[[bglS]]''
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yxiS]]'', ''[[citH]]''
 
|-
 
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB15932&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
+
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB15931&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
 
|-
 
|-
 
|-
 
|-
 
|-
 
|-
|colspan="2" | '''Genetic context''' <br/> [[Image:citH_context.gif]]
+
|colspan="2" | '''Genetic context''' <br/> [[Image:katE_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|-
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__TOC__
 
__TOC__
  
<br/><br/>
+
<br/><br/><br/><br/>
  
 
=The gene=
 
=The gene=
Line 43: Line 43:
 
=== Database entries ===
 
=== Database entries ===
  
* '''DBTBS entry:''' no entry
+
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/katE-yxiS.html]
  
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11146]
+
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11102]
  
 
=== Additional information===
 
=== Additional information===
Line 66: Line 66:
 
* '''Domains:'''  
 
* '''Domains:'''  
  
* '''Modification:''' phosphorylation on Ser-149 [http://www.ncbi.nlm.nih.gov/sites/entrez/17218307 PubMed]
+
* '''Modification:'''
  
 
* '''Cofactor(s):'''
 
* '''Cofactor(s):'''
Line 82: Line 82:
 
* '''Swiss prot entry:'''
 
* '''Swiss prot entry:'''
  
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU39060]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU39050]
  
 
* '''E.C. number:'''
 
* '''E.C. number:'''
Line 92: Line 92:
 
* '''Operon:'''  
 
* '''Operon:'''  
  
* '''[[Sigma factor]]:'''  
+
* '''[[Sigma factor]]:''' [[SigB]] [http://www.ncbi.nlm.nih.gov/sites/entrez/7559348 PubMed]
  
* '''Regulation:'''  
+
* '''Regulation:''' induced by stress ([[SigB]]) [http://www.ncbi.nlm.nih.gov/pubmed/11544224 PubMed]
  
 
* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
  
* '''Additional information:'''  
+
* '''Additional information:'''
  
 
=Biological materials =
 
=Biological materials =
Line 120: Line 120:
 
=References=
 
=References=
  
# Macek et al. (2007) The serine/ threonine/ tyrosine phosphoproteome of the model  bacterium ''Bacillus subtilis''. Mol. Cell. Proteomics 6: 697-707  [http://www.ncbi.nlm.nih.gov/pubmed/17218307 PubMed]
+
# Petersohn et al. (2001) Global Analysis of the General Stress Response of ''Bacillus subtilis''. ''J Bacteriol.'' '''183:''' 5617-5631 [http://www.ncbi.nlm.nih.gov/pubmed/11544224 PubMed]
 +
# Engelmann, S., Lindner, C. and Hecker, M. 1995. Cloning, nucleotide sequence, and regulation of ''katE'' encoding a (sigma)B-dependent catalase in ''Bacillus subtilis''. J. Bacteriol. 177: 5598-5605. [http://www.ncbi.nlm.nih.gov/sites/entrez/7559348 PubMed]
 
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]
 
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]

Revision as of 23:52, 16 April 2009

  • Description: catalase, general stress protein

Gene name katE
Synonyms katB
Essential no
Product catalase 2
Function detoxification (degradation) of hydrogen peroxide
MW, pI 77 kDa, 5.845
Gene length, protein length 2058 bp, 686 aa
Immediate neighbours yxiS, citH
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
KatE context.gif
This image was kindly provided by SubtiList





The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • Swiss prot entry:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Petersohn et al. (2001) Global Analysis of the General Stress Response of Bacillus subtilis. J Bacteriol. 183: 5617-5631 PubMed
  2. Engelmann, S., Lindner, C. and Hecker, M. 1995. Cloning, nucleotide sequence, and regulation of katE encoding a (sigma)B-dependent catalase in Bacillus subtilis. J. Bacteriol. 177: 5598-5605. PubMed
  3. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed