Difference between revisions of "Sandbox"

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* '''Description:''' unknown <br/><br/>
+
* '''Description:''' rRNA adenine dimethyltransferase <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''yabG''
+
|''ksgA''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
 
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
Line 10: Line 10:
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || unknown
+
|style="background:#ABCDEF;" align="center"| '''Product''' || rRNA adenine dimethyltransferase
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || unknown
+
|style="background:#ABCDEF;" align="center"|'''Function''' || resistance to kasugamycin
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 33 kDa, 9.143  
+
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 32 kDa, 5.712  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 870 bp, 290 aa  
+
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 876 bp, 292 aa  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ksgA]]'', ''[[veg]]''
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[rnmV]]'', ''[[yabG]]''
 
|-
 
|-
 
|colspan="2" style="background:#FAF8CC;" align="center"|'''Hier soll was neues rein'''
 
|colspan="2" style="background:#FAF8CC;" align="center"|'''Hier soll was neues rein'''
 
|-
 
|-
|colspan="2" | '''Genetic context''' <br/> [[Image:yabG_context.gif]]
+
|colspan="2" | '''Genetic context''' <br/> [[Image:ksgA_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|-
Line 41: Line 41:
 
=== Database entries ===
 
=== Database entries ===
  
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/yabG.html]
+
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/rnmV-ksgA.html]
  
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10106]
+
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10105]
  
 
=== Additional information===
 
=== Additional information===
Line 72: Line 72:
 
* '''Interactions:'''
 
* '''Interactions:'''
  
* '''Localization:''' spore coat [http://www.ncbi.nlm.nih.gov/sites/entrez/19060142 PubMed]
+
* '''Localization:'''
  
 
=== Database entries ===
 
=== Database entries ===
Line 80: Line 80:
 
* '''Swiss prot entry:'''
 
* '''Swiss prot entry:'''
  
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU00430]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU00420]
  
 
* '''E.C. number:'''
 
* '''E.C. number:'''
  
 
=== Additional information===
 
=== Additional information===
colocalizes in the spore coat with [[YeeK]] [http://www.ncbi.nlm.nih.gov/sites/entrez/19060142 PubMed]
 
  
 
=Expression and regulation=
 
=Expression and regulation=
  
* '''Operon:''' ''yabG'' [http://www.ncbi.nlm.nih.gov/sites/entrez/10714992 PubMed]
+
* '''Operon:''' ''[[rnmV]]-[[ksgA]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/11233981 PubMed]  
  
* '''[[Sigma factor]]:''' [[SigK]] [http://www.ncbi.nlm.nih.gov/sites/entrez/10714992 PubMed]
+
* '''[[Sigma factor]]:'''  
  
* '''Regulation:''' expression starts four hours after onset of sporulation [http://www.ncbi.nlm.nih.gov/sites/entrez/10714992 PubMed]
+
* '''Regulation:'''  
  
 
* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
Line 119: Line 118:
 
=References=
 
=References=
  
# Takamatsu ''et al''. (2000) The ''Bacillus subtilis yabG'' gene is transcribed by SigK RNA polymerase during sporulation, and yabG mutant spores have altered coat protein composition.''J. Bacteriol.'' '''182:''' 1883-1888. [http://www.ncbi.nlm.nih.gov/sites/entrez/10714992 PubMed]
+
# Condon et al. (2001) Identification of the gene encoding the 5S ribosomal RNA maturase in ''Bacillus subtilis'': mature 5S rRNA is dispensable for ribosome function. ''RNA'' '''7:''' 242-253. [http://www.ncbi.nlm.nih.gov/sites/entrez/11233981 PubMed]
 +
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]

Revision as of 21:41, 15 April 2009

  • Description: rRNA adenine dimethyltransferase

Gene name ksgA
Synonyms
Essential no
Product rRNA adenine dimethyltransferase
Function resistance to kasugamycin
MW, pI 32 kDa, 5.712
Gene length, protein length 876 bp, 292 aa
Immediate neighbours rnmV, yabG
Hier soll was neues rein
Genetic context
KsgA context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • Swiss prot entry:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Condon et al. (2001) Identification of the gene encoding the 5S ribosomal RNA maturase in Bacillus subtilis: mature 5S rRNA is dispensable for ribosome function. RNA 7: 242-253. PubMed
  2. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed