Difference between revisions of "Sandbox"

From SubtiWiki
Jump to: navigation, search
Line 1: Line 1:
−
* '''Description:''' 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase <br/><br/>
+
* '''Description:''' small acid-soluble spore protein (minor) <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
−
|''ispE''
+
|''sspF''
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yabH ''
+
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
+
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''Product''' || 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
+
|style="background:#ABCDEF;" align="center"| '''Product''' || small acid-soluble spore protein (minor)
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"|'''Function''' || isoprenoid biosynthesis
+
|style="background:#ABCDEF;" align="center"|'''Function''' || protection of spore DNA
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 31 kDa, 6.349  
+
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 6 kDa, 9.647  
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 867 bp, 289 aa  
+
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 183 bp, 61 aa  
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[sspF]]'', ''[[purR]]''
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[veg]]'', ''[[ispE]]''
 
|-
 
|-
 
|colspan="2" style="background:#FAF8CC;" align="center"|'''Hier soll was neues rein'''
 
|colspan="2" style="background:#FAF8CC;" align="center"|'''Hier soll was neues rein'''
 
|-
 
|-
−
|colspan="2" | '''Genetic context''' <br/> [[Image:ispE_context.gif]]
+
|colspan="2" | '''Genetic context''' <br/> [[Image:sspF_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|-
Line 38: Line 38:
  
 
===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
−
 
−
essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
 
  
 
=== Database entries ===
 
=== Database entries ===
  
−
* '''DBTBS entry:''' no entry
+
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/sspF.html]
  
−
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10109]
+
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10108]
  
 
=== Additional information===
 
=== Additional information===
Line 82: Line 80:
 
* '''Swiss prot entry:'''
 
* '''Swiss prot entry:'''
  
−
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU00460]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU00450]
  
 
* '''E.C. number:'''
 
* '''E.C. number:'''
Line 90: Line 88:
 
=Expression and regulation=
 
=Expression and regulation=
  
−
* '''Operon:'''  
+
* '''Operon:''' ''sspF'' [http://www.ncbi.nlm.nih.gov/sites/entrez/6205155 PubMed]
  
−
* '''[[Sigma factor]]:'''  
+
* '''[[Sigma factor]]:''' [[SigG]] [http://www.ncbi.nlm.nih.gov/sites/entrez/6205155 PubMed]
  
−
* '''Regulation:'''  
+
* '''Regulation:''' switched on during stage  III of sporulation [http://www.ncbi.nlm.nih.gov/sites/entrez/6205155 PubMed]
  
 
* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
  
−
* '''Additional information:'''  
+
* '''Additional information:'''
  
 
=Biological materials =
 
=Biological materials =
Line 120: Line 118:
 
=References=
 
=References=
  
−
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]
+
# Stephens et al. (1984) A promoter whose utilization is temporally regulated during sporulation in ''Bacillus subtilis''. ''J. Mol. Biol.'' '''176:''' 333-348. [http://www.ncbi.nlm.nih.gov/sites/entrez/6205155 PubMed]

Revision as of 21:41, 15 April 2009

  • Description: small acid-soluble spore protein (minor)

Gene name sspF
Synonyms
Essential no
Product small acid-soluble spore protein (minor)
Function protection of spore DNA
MW, pI 6 kDa, 9.647
Gene length, protein length 183 bp, 61 aa
Immediate neighbours veg, ispE
Hier soll was neues rein
Genetic context
SspF context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • Swiss prot entry:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation: switched on during stage III of sporulation PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Stephens et al. (1984) A promoter whose utilization is temporally regulated during sporulation in Bacillus subtilis. J. Mol. Biol. 176: 333-348. PubMed
Retrieved from "http://subtiwiki.uni-goettingen.de/wiki//index.php?title=Sandbox&oldid=57154"