Difference between revisions of "Sandbox"

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* '''Description:''' transcription repressor of the pur operon <br/><br/>
+
* '''Description:''' 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''purR''
+
|''ispE''
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yabI ''
+
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yabH ''
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
+
|style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || transcription repressor of the pur operon
+
|style="background:#ABCDEF;" align="center"| '''Product''' || 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || regulation of purine biosynthesis
+
|style="background:#ABCDEF;" align="center"|'''Function''' || isoprenoid biosynthesis  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 31 kDa, 5.771  
+
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 31 kDa, 6.349  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 855 bp, 285 aa  
+
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 867 bp, 289 aa  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ispE]]'', ''[[yabJ]]''
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[sspF]]'', ''[[purR]]''
 
|-
 
|-
 
|colspan="2" style="background:#FAF8CC;" align="center"|'''Hier soll was neues rein'''
 
|colspan="2" style="background:#FAF8CC;" align="center"|'''Hier soll was neues rein'''
 
|-
 
|-
|colspan="2" | '''Genetic context''' <br/> [[Image:purR_context.gif]]
+
|colspan="2" | '''Genetic context''' <br/> [[Image:ispE_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|-
Line 38: Line 38:
  
 
===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
 +
 +
essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
  
 
=== Database entries ===
 
=== Database entries ===
  
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/purR-yabJ.html]
+
* '''DBTBS entry:''' no entry
  
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10110]
+
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10109]
  
 
=== Additional information===
 
=== Additional information===
Line 52: Line 54:
 
=== Basic information/ Evolution ===
 
=== Basic information/ Evolution ===
  
* '''Biological activity:''' transcriptional repressor of purine biosynthetic operons
+
* '''Catalyzed reaction/ biological activity:'''  
  
 
* '''Protein family:'''
 
* '''Protein family:'''
Line 68: Line 70:
 
* '''Cofactor(s):'''
 
* '''Cofactor(s):'''
  
* '''Effectors of protein activity:''' PRPP acts as inducer, binding results in release of PurR from the operator [http://www.ncbi.nlm.nih.gov/sites/entrez/10919400 PubMed]
+
* '''Effectors of protein activity:'''
  
* '''Interactions:'''  
+
* '''Interactions:'''
  
 
* '''Localization:'''
 
* '''Localization:'''
Line 76: Line 78:
 
=== Database entries ===
 
=== Database entries ===
  
* '''Structure:''' 1P4A (complex with PRPP) [http://www.ncbi.nlm.nih.gov/Structure/mmdb/mmdbsrv.cgi?uid=25670 NCBI] [http://www.ncbi.nlm.nih.gov/sites/entrez/12837783 PubMed]
+
* '''Structure:'''
  
 
* '''Swiss prot entry:'''
 
* '''Swiss prot entry:'''
  
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU00470]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU00460]
  
 
* '''E.C. number:'''
 
* '''E.C. number:'''
  
 
=== Additional information===
 
=== Additional information===
 
requires [[YabJ]] for ''in vivo'' activity
 
  
 
=Expression and regulation=
 
=Expression and regulation=
  
* '''Operon:''' ''[[purR]]-[[yabJ]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/7638212 PubMed]
+
* '''Operon:'''  
  
 
* '''[[Sigma factor]]:'''  
 
* '''[[Sigma factor]]:'''  
  
* '''Regulation:''' negative autoregulation [http://www.ncbi.nlm.nih.gov/sites/entrez/7638212 PubMed]
+
* '''Regulation:'''  
  
* '''Regulatory mechanism:''' repression by [[PurR]] [http://www.ncbi.nlm.nih.gov/sites/entrez/7638212 PubMed]
+
* '''Regulatory mechanism:'''  
  
 
* '''Additional information:'''  
 
* '''Additional information:'''  
Line 120: Line 120:
 
=References=
 
=References=
  
# Weng et al. (1995) Identification of the ''Bacillus subtilis pur'' operon repressor. ''Proc. Natl. Acad. Sci. USA'' '''92:''' 7455-7459. [http://www.ncbi.nlm.nih.gov/sites/entrez/7638212 PubMed]
 
# Weng & Zalkin (2000) Mutations in the ''Bacillus subtilis'' purine repressor that perturb PRPP effector function ''in vitro'' and ''in vivo''.''Currr. Microbiol.'' '''41:''' 56-59. [http://www.ncbi.nlm.nih.gov/sites/entrez/10919400 PubMed]
 
# Sinha et al. (2003) The purine repressor of ''Bacillus subtilis'': a novel combination of domains adapted for transcription regulation.''J. Bacteriol.'' '''185:''' 4087-4098. [http://www.ncbi.nlm.nih.gov/sites/entrez/12837783 PubMed]
 
 
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]
 
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]

Revision as of 21:41, 15 April 2009

  • Description: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Gene name ispE
Synonyms yabH
Essential yes PubMed
Product 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
Function isoprenoid biosynthesis
MW, pI 31 kDa, 6.349
Gene length, protein length 867 bp, 289 aa
Immediate neighbours sspF, purR
Hier soll was neues rein
Genetic context
IspE context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • Swiss prot entry:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed