Difference between revisions of "Sandbox"

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* '''Description:''' two-component sensor kinase, regulation of citrate uptake <br/><br/>
+
* '''Description:''' malate transporter <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''citS''
+
|''yflS''
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yflR ''
+
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || two-component sensor kinase
+
|style="background:#ABCDEF;" align="center"| '''Product''' || malate transporter
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || regulation of citrate uptake
+
|style="background:#ABCDEF;" align="center"|'''Function''' || malate uptake
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 59 kDa, 6.481  
+
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 51 kDa, 9.741  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1626 bp, 542 aa  
+
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1434 bp, 478 aa  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yflS]]'', ''[[citT]]''
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[pel]]'', ''[[citS]]''
 
|-
 
|-
 
|colspan="2" style="background:#FAF8CC;" align="center"|'''Hier soll was neues rein'''
 
|colspan="2" style="background:#FAF8CC;" align="center"|'''Hier soll was neues rein'''
 
|-
 
|-
|colspan="2" | '''Genetic context''' <br/> [[Image:citS_context.gif]]
+
|colspan="2" | '''Genetic context''' <br/> [[Image:yflS_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|-
Line 41: Line 41:
 
=== Database entries ===
 
=== Database entries ===
  
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/citST-yflP.html]
+
* '''DBTBS entry:''' no entry
  
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12576]
+
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12951]
  
 
=== Additional information===
 
=== Additional information===
Line 62: Line 62:
 
* '''Kinetic information:'''
 
* '''Kinetic information:'''
  
* '''Domains:''' two transmembrane segments, C-terminal histidine phosphotransferase domain
+
* '''Domains:'''  
  
 
* '''Modification:'''
 
* '''Modification:'''
Line 72: Line 72:
 
* '''Interactions:'''
 
* '''Interactions:'''
  
* '''Localization:''' Membrane (Heterogeneous) [http://www.ncbi.nlm.nih.gov/sites/entrez/16479537 PubMed]
+
* '''Localization:'''
  
 
=== Database entries ===
 
=== Database entries ===
Line 80: Line 80:
 
* '''Swiss prot entry:'''
 
* '''Swiss prot entry:'''
  
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU07580]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU07570]
  
 
* '''E.C. number:'''
 
* '''E.C. number:'''
Line 92: Line 92:
 
* '''[[Sigma factor]]:'''  
 
* '''[[Sigma factor]]:'''  
  
* '''Regulation:'''  
+
* '''Regulation:''' induced by malate ([[MalR]])
  
* '''Regulatory mechanism:'''  
+
* '''Regulatory mechanism:''' [[MalR]]: transcription activation
  
* '''Additional information:'''  
+
* '''Additional information:'''
  
 
=Biological materials =
 
=Biological materials =
Line 118: Line 118:
 
=References=
 
=References=
  
# Meile et al. (2006) Systematic localisation of proteins fused to the green fluorescent protein in ''Bacillus subtilis'': identification of new proteins at the DNA replication factory ''Proteomics'' '''6:''' 2135-2146. [http://www.ncbi.nlm.nih.gov/sites/entrez/16479537 PubMed]
+
# Tanaka, K., K. Kobayashi, and N. Ogasawara. 2003. The ''Bacillus subtilis'' YufLM two-component system regulates the expression of the malate transporters MaeN (YufR) and YflS, and is essential for utilization of malate in minimal medium. Microbiology 149: 2317-2329. [http://www.ncbi.nlm.nih.gov/sites/entrez/12949159 PubMed]
 
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]
 
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]

Revision as of 20:52, 15 April 2009

  • Description: malate transporter

Gene name yflS
Synonyms
Essential no
Product malate transporter
Function malate uptake
MW, pI 51 kDa, 9.741
Gene length, protein length 1434 bp, 478 aa
Immediate neighbours pel, citS
Hier soll was neues rein
Genetic context
YflS context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • Swiss prot entry:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation: induced by malate (MalR)
  • Regulatory mechanism: MalR: transcription activation
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Tanaka, K., K. Kobayashi, and N. Ogasawara. 2003. The Bacillus subtilis YufLM two-component system regulates the expression of the malate transporters MaeN (YufR) and YflS, and is essential for utilization of malate in minimal medium. Microbiology 149: 2317-2329. PubMed
  2. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed