Difference between revisions of "Sandbox"

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* '''Description:''' ribulose 5-phosphate 3-epimerase <br/><br/>
+
* '''Description:''' activity stimulated by ribosomes <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''rpe''
+
|''cpgA ''
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yloR ''
+
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yloQ''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || ribulose 5-phosphate 3-epimerase
+
|style="background:#ABCDEF;" align="center"| '''Product''' || GTPase
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || pentose phosphate pathway
+
|style="background:#ABCDEF;" align="center"|'''Function''' || unknown
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 23 kDa, 5.651  
+
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 33 kDa, 4.743  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 651 bp, 217 aa  
+
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 894 bp, 298 aa  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[cpgA]]'', ''[[yloS]]''
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[prkC]]'', ''[[rpe]]''
 
|-
 
|-
 
|colspan="2" style="background:#FAF8CC;" align="center"|'''Hier soll was neues rein'''
 
|colspan="2" style="background:#FAF8CC;" align="center"|'''Hier soll was neues rein'''
 
|-
 
|-
|colspan="2" | '''Genetic context''' <br/> [[Image:rpe_context.gif]]
+
|colspan="2" | '''Genetic context''' <br/> [[Image:yloQ_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|-
Line 43: Line 43:
 
* '''DBTBS entry:''' no entry
 
* '''DBTBS entry:''' no entry
  
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13393]
+
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13392]
  
 
=== Additional information===
 
=== Additional information===
Line 64: Line 64:
 
* '''Domains:'''  
 
* '''Domains:'''  
  
* '''Modification:'''
+
* '''Modification:''' phosphorylated on Ser/ Thr by [[PrkC]], dephosphorylated by [[PrpC]]  [http://www.ncbi.nlm.nih.gov/sites/entrez/19246764 PubMed]
  
 
* '''Cofactor(s):'''
 
* '''Cofactor(s):'''
Line 80: Line 80:
 
* '''Swiss prot entry:'''
 
* '''Swiss prot entry:'''
  
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU15790]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU15780]
  
 
* '''E.C. number:'''
 
* '''E.C. number:'''
Line 113: Line 113:
  
 
=Labs working on this gene/protein=
 
=Labs working on this gene/protein=
 +
 +
[[Tony Wilkinson]], York University, U.K. [http://www.york.ac.uk/depts/chem/staff/ajw.html homepage]
  
 
=Your additional remarks=
 
=Your additional remarks=
Line 118: Line 120:
 
=References=
 
=References=
  
 +
# Hunt, A., Rawlins, J. P., Thomaides, H. B., and Errington, J. (2006) Functional analysis of 11 putative essential genes in Bacillus subtilis. Microbiology 152, 2895-2907. [http://www.ncbi.nlm.nih.gov/sites/entrez/17005971 PubMed]
 +
# Absalon C, Obuchowski M, Madec E, Delattre D, Holland IB, Séror SJ (2009) CpgA, EF-Tu and the stressosome protein YezB are substrates of the Ser/Thr kinase/phosphatase couple, PrkC/PrpC, in ''Bacillus subtilis''. ''Microbiology'' '''155:''' 932-943. [http://www.ncbi.nlm.nih.gov/sites/entrez/19246764 PubMed]
 +
# Absalon C, Hamze K, Blanot D, Frehel C, Carballido-Lopez R, Holland BI, van Heijenoort J, Séror SJ. (2008) The GTPase CpgA is implicated in the deposition of the peptidoglycan sacculus in Bacillus subtilis.  ''J Bacteriol.'' '''May;190(10):''' 3786-90. [http://www.ncbi.nlm.nih.gov/sites/entrez/18344364 PubMed]
 
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]
 
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]

Revision as of 19:53, 15 April 2009

  • Description: activity stimulated by ribosomes

Gene name cpgA
Synonyms yloQ
Essential no
Product GTPase
Function unknown
MW, pI 33 kDa, 4.743
Gene length, protein length 894 bp, 298 aa
Immediate neighbours prkC, rpe
Hier soll was neues rein
Genetic context
YloQ context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification: phosphorylated on Ser/ Thr by PrkC, dephosphorylated by PrpC PubMed
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • Swiss prot entry:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Tony Wilkinson, York University, U.K. homepage

Your additional remarks

References

  1. Hunt, A., Rawlins, J. P., Thomaides, H. B., and Errington, J. (2006) Functional analysis of 11 putative essential genes in Bacillus subtilis. Microbiology 152, 2895-2907. PubMed
  2. Absalon C, Obuchowski M, Madec E, Delattre D, Holland IB, Séror SJ (2009) CpgA, EF-Tu and the stressosome protein YezB are substrates of the Ser/Thr kinase/phosphatase couple, PrkC/PrpC, in Bacillus subtilis. Microbiology 155: 932-943. PubMed
  3. Absalon C, Hamze K, Blanot D, Frehel C, Carballido-Lopez R, Holland BI, van Heijenoort J, Séror SJ. (2008) The GTPase CpgA is implicated in the deposition of the peptidoglycan sacculus in Bacillus subtilis. J Bacteriol. May;190(10): 3786-90. PubMed
  4. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed