Difference between revisions of "Sandbox"

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* '''Description:''' tryptophan operon RNA-binding attenuation protein (TRAP) <br/><br/>
+
* '''Description:''' heptaprenyl diphosphate synthase component I <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
−
|''mtrB''
+
|''hepS''
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
+
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''gerCA, hepA ''
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
+
|style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''Product''' || tryptophan operon RNA-binding attenuation protein (TRAP)
+
|style="background:#ABCDEF;" align="center"| '''Product''' || heptaprenyl diphosphate synthase component I
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"|'''Function''' || regulation of tryptophan biosynthesis
+
|style="background:#ABCDEF;" align="center"|'''Function''' || menaquinone biosynthesis
−
(and translation) attenuation in the trp operon);
 
−
repression of the folate operon
 
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 8 kDa, 7.333  
+
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 28 kDa, 6.273  
 
|-
 
|-
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|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 225 bp, 75 aa  
+
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 753 bp, 251 aa  
 
|-
 
|-
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[hepS]]'', ''[[mtrA]]''
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[menH]]'', ''[[mtrB]]''
 
|-
 
|-
 
|colspan="2" style="background:#FAF8CC;" align="center"|'''Hier soll was neues rein'''
 
|colspan="2" style="background:#FAF8CC;" align="center"|'''Hier soll was neues rein'''
 
|-
 
|-
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|colspan="2" | '''Genetic context''' <br/> [[Image:mtrB_context.gif]]
+
|colspan="2" | '''Genetic context''' <br/> [[Image:hepS_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|-
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===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
 +
 +
essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
  
 
=== Database entries ===
 
=== Database entries ===
  
−
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/mtrAB.html]
+
* '''DBTBS entry:''' no entry
  
−
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10278]
+
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10279]
  
 
=== Additional information===
 
=== Additional information===
Line 82: Line 82:
 
* '''Swiss prot entry:'''
 
* '''Swiss prot entry:'''
  
−
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU22770]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU22760]
  
 
* '''E.C. number:'''
 
* '''E.C. number:'''
Line 120: Line 120:
 
=References=
 
=References=
  
−
# Antson AA, Otridge J, Brzozowski AM, Dodson EJ, Dodson GG, Wilson KS, Smith TM, Yang M, Kurecki T, Gollnick P (1995) The structure of ''trp'' RNA-binding protein. Nature 374:693-700. [http://www.ncbi.nlm.nih.gov/sites/entrez/7715723 PubMed]
 
−
# Antson AA, Dodson EJ, Dodson G, Greaves RB, Chen XP, Gollnick P (1999) Structure of the ''trp'' RNA-binding attenuation protein, TRAP, bound to RNA. Nature 401:235-242. [http://www.ncbi.nlm.nih.gov/sites/entrez/10499579 PubMed]
 
−
# Babitzke P, Gollnick P (2001) Posttransription iniation control of tryptophan metabolism in ''Bacillus subtilis'' by the trp RNA-binding attenuation protein (TRAP), anti-TRAP, and RNA structure. J Bacteriol 183:5795-5802. [http://www.ncbi.nlm.nih.gov/sites/entrez/11566976 PubMed]
 
−
# Babitzke P, Stults JT, Shire SJ, Yanofsky C (1994) TRAP, the trp RNA-binding attenuation protein of ''Bacillus subtilis'', is a multisubunit complex that appears  to recognize G/UAG repeats in the ''trpEDCFBA'' and ''trpG'' transcripts. J Biol Chem 269:16597-16604. [http://www.ncbi.nlm.nih.gov/sites/entrez/7515880 PubMed]
 
 
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]
 
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]

Revision as of 18:59, 15 April 2009

  • Description: heptaprenyl diphosphate synthase component I

Gene name hepS
Synonyms gerCA, hepA
Essential yes PubMed
Product heptaprenyl diphosphate synthase component I
Function menaquinone biosynthesis
MW, pI 28 kDa, 6.273
Gene length, protein length 753 bp, 251 aa
Immediate neighbours menH, mtrB
Hier soll was neues rein
Genetic context
HepS context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • Swiss prot entry:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed
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