Difference between revisions of "Sandbox"

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* '''Description:''' MreD is a cell shape determining protein and is associated with the MreB cytoskeleton in B. subtilis and other rod shaped bacteria. <br/><br/>
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* '''Description:''' cell-division inhibitor (septum placement), destabilizes FtsZ-rings at new cell poles <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
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|''mreD''
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|''minC''
 
|-
 
|-
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|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''rodB ''
+
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''Essential''' || yes
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|style="background:#ABCDEF;" align="center"| '''Essential''' || no
 
|-
 
|-
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|style="background:#ABCDEF;" align="center"| '''Product''' || cell-shape determining protein
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|style="background:#ABCDEF;" align="center"| '''Product''' || cell-division inhibitor
 
|-
 
|-
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|style="background:#ABCDEF;" align="center"|'''Function''' || cell-shape determation
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|style="background:#ABCDEF;" align="center"|'''Function''' || septum placement
 
|-
 
|-
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 19 kDa, 7.906  
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 24 kDa, 6.262  
 
|-
 
|-
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|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 516 bp, 172 aa  
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|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 678 bp, 226 aa  
 
|-
 
|-
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[minC]]'', ''[[mreC]]''
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[minD]]'', ''[[mreD]]''
 
|-
 
|-
 
|colspan="2" style="background:#FAF8CC;" align="center"|'''Hier soll was neues rein'''
 
|colspan="2" style="background:#FAF8CC;" align="center"|'''Hier soll was neues rein'''
 
|-
 
|-
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|colspan="2" | '''Genetic context''' <br/> [[Image:mreD_context.gif]]
+
|colspan="2" | '''Genetic context''' <br/> [[Image:minC_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|-
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=== Basic information ===
 
=== Basic information ===
  
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* '''Coordinates:''' 2858547-2859062
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* '''Coordinates:'''
  
 
===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
−
 
−
the phenotype of ''mreD'' is similar to that of ''[[mreC]]''.  ''mreD'' is essential under normal conditions [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed].  Depletion of MreD leads to a progressive increase in the width and a decrease in the length of the cell and cells become lytic. In the depletion strain, lysis can be prevented and cell growth, but not cell shape, can be recovered by inculaion of Magnesium in the media.  This shape defect is consistent with a role for ''mreD'' in cell wall synthesis during elongation and has a similar phenotype to other genes with roles in elongation.
 
−
 
  
 
=== Database entries ===
 
=== Database entries ===
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* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/mreBCD-minCD.html]
 
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/mreBCD-minCD.html]
  
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* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10328]
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* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10329]
  
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= Additional information=
+
=== Additional information===
  
−
 
−
===Function===
 
−
 
−
MreD functions in cell wall synthesis by, together with the MreB cytoskeleton, localizing the cell wall synthetic machinery to the correct part of the cell.  As a transmembrane protein MreD is thought to provide a patch on the membrane that MreB inteacts with.  MreC therefore ensures that the cell wall is made in the correct way to maintain the proper shape of the cell.
 
−
 
−
===''mreD'' and the mapping of ''mre-min'' operon===
 
−
 
−
The ''mre-min'' operon was first sequenced by levin et al in 1992.  The temperature sentitive ''rodB1'' mutation mapperd to that region.
 
  
 
=The protein=
 
=The protein=
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=== Basic information/ Evolution ===
 
=== Basic information/ Evolution ===
  
−
* '''Catalyzed reaction/ biological activity:''' None/ structural
+
* '''Catalyzed reaction/ biological activity:'''  
  
−
* '''Protein family:''' COG2891
+
* '''Protein family:'''
  
−
* '''Paralogous protein(s):''' None
+
* '''Paralogous protein(s):'''
  
 
=== Extended information on the protein ===
 
=== Extended information on the protein ===
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=== Database entries ===
 
=== Database entries ===
  
−
* '''Structure:''' None
+
* '''Structure:'''
  
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* '''Swiss prot entry:''' Q01467
+
* '''Swiss prot entry:'''
  
−
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU28010]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU28000]
  
 
* '''E.C. number:'''
 
* '''E.C. number:'''
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=Expression and regulation=
 
=Expression and regulation=
  
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* '''Operon:''' ''[[mreB]]''-''[[mreC]]''-'''mreD'''-''[[minC]]''-''[[minD]]''
+
* '''Operon:'''  
  
 
* '''[[Sigma factor]]:''' [[SigH]] [http://www.ncbi.nlm.nih.gov/sites/entrez/8459776 PubMed]
 
* '''[[Sigma factor]]:''' [[SigH]] [http://www.ncbi.nlm.nih.gov/sites/entrez/8459776 PubMed]
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=Biological materials =
 
=Biological materials =
  
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* '''Mutant:''' A conditional mutant with an inframe deletion of ''mreD'' complemented by a xylose inducible copy at an ectopic locus (strain named 4352) is avaliable from the Errington Group.
+
* '''Mutant:'''
  
 
* '''Expression vector:'''
 
* '''Expression vector:'''
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* '''lacZ fusion:'''
 
* '''lacZ fusion:'''
  
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* '''GFP fusion:''' A functional N-terminal GFP fusion has been made where the fusion protein is the only copy of the gene in the cell: strain 3416 [http://www.ncbi.nlm.nih.gov/pubmed/16101995 PubMed].
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* '''GFP fusion:'''
−
 
 
  
 
* '''two-hybrid system:'''  
 
* '''two-hybrid system:'''  
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=Labs working on this gene/protein=
 
=Labs working on this gene/protein=
−
 
−
[[Peter Graumann]], Freiburg University, Germany [http://www.biologie.uni-freiburg.de/data/bio2/graumann/index.htm homepage]
 
  
 
=Your additional remarks=
 
=Your additional remarks=

Revision as of 18:26, 15 April 2009

  • Description: cell-division inhibitor (septum placement), destabilizes FtsZ-rings at new cell poles

Gene name minC
Synonyms
Essential no
Product cell-division inhibitor
Function septum placement
MW, pI 24 kDa, 6.262
Gene length, protein length 678 bp, 226 aa
Immediate neighbours minD, mreD
Hier soll was neues rein
Genetic context
MinC context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • Swiss prot entry:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed
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