Difference between revisions of "Sandbox"

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* '''Description:''' uric acid permease <br/><br/>
+
* '''Description:''' transcriptional regulator of puc genes <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''pucJ''
+
|''pucR''
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yunJ ''
+
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yunI ''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || uric acid permease
+
|style="background:#ABCDEF;" align="center"| '''Product''' || transcriptional regulator of puc genes
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || purine utilization
+
|style="background:#ABCDEF;" align="center"|'''Function''' || regulation of purine utilization
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 46 kDa, 9.638  
+
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 60 kDa, 6.188  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1347 bp, 449 aa  
+
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1593 bp, 531 aa  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[pucR]]'', ''[[pucK]]''
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[pucH]]'', ''[[pucJ]]''
 
|-
 
|-
 
|colspan="2" style="background:#FAF8CC;" align="center"|'''Hier soll was neues rein'''
 
|colspan="2" style="background:#FAF8CC;" align="center"|'''Hier soll was neues rein'''
 
|-
 
|-
|colspan="2" | '''Genetic context''' <br/> [[Image:pucJ_context.gif]]
+
|colspan="2" | '''Genetic context''' <br/> [[Image:pucR_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|-
Line 41: Line 41:
 
=== Database entries ===
 
=== Database entries ===
  
* '''DBTBS entry:''' no entry
+
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/pucRJKLM.html]
  
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13984]
+
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13983]
  
 
=== Additional information===
 
=== Additional information===
Line 80: Line 80:
 
* '''Swiss prot entry:'''
 
* '''Swiss prot entry:'''
  
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU32430]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU32420]
  
 
* '''E.C. number:'''
 
* '''E.C. number:'''
Line 92: Line 92:
 
* '''[[Sigma factor]]:'''  
 
* '''[[Sigma factor]]:'''  
  
* '''Regulation:'''  
+
* '''Regulation:''' negative autoregulation [http://www.ncbi.nlm.nih.gov/sites/entrez/12029039 PubMed]
  
* '''Regulatory mechanism:'''  
+
* '''Regulatory mechanism:''' repression by '''PucR''' [http://www.ncbi.nlm.nih.gov/sites/entrez/12029039 PubMed]
  
* '''Additional information:'''  
+
* '''Additional information:'''
  
 
=Biological materials =
 
=Biological materials =

Revision as of 17:55, 15 April 2009

  • Description: transcriptional regulator of puc genes

Gene name pucR
Synonyms yunI
Essential no
Product transcriptional regulator of puc genes
Function regulation of purine utilization
MW, pI 60 kDa, 6.188
Gene length, protein length 1593 bp, 531 aa
Immediate neighbours pucH, pucJ
Hier soll was neues rein
Genetic context
PucR context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • Swiss prot entry:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation: negative autoregulation PubMed
  • Regulatory mechanism: repression by PucR PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed