Difference between revisions of "Sandbox"

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* '''Description:''' Sda is a checkpoint protein that is upregulated in response to replication stress [http://www.ncbi.nlm.nih.gov/sites/entrez?db=pubmed&cmd=DetailsSearch&term=11207367%5Buid%5D&log$=activity (Burkholder et al 2001, Cell)]. Sda inhibits the autokinase activity of [http://subtiwiki.uni-goettingen.de/wiki/index.php/KinA KinA] (and likely [http://subtiwiki.uni-goettingen.de/wiki/index.php/KinB KinB] as well). This in its turn results in reduced levels of [http://subtiwiki.uni-goettingen.de/wiki/index.php/Spo0A Spo0A~P]. Thus, replication stressed cells will not iniate sporulation<br/><br/> Sda is rapidly turned over by the [http://subtiwiki.uni-goettingen.de/wiki/index.php/ClpP ClpP/X] system because of some uncharged residues at its C-terminus [http://www.ncbi.nlm.nih.gov/sites/entrez?db=pubmed&cmd=DetailsSearch&term=16796683%5Buid%5D&log$=activity Ruvolo et al 2006, MolMic].
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* '''Description:''' general stress protein, transcriptional activator of the bmrU-bmrR operon <br/><br/>
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{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
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|''sda''
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|''bmrR''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
 
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
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|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
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|style="background:#ABCDEF;" align="center"| '''Product''' || developmental checkpoint protein
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|style="background:#ABCDEF;" align="center"| '''Product''' || transcriptional activator
 
|-
 
|-
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|style="background:#ABCDEF;" align="center"|'''Function''' || mediates a developmental checkpoint coupling initiation of
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|style="background:#ABCDEF;" align="center"|'''Function''' || regulation of multidrug resistance
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sporulation (phosphorylation of Spo0A) to the function
 
−
 
 
−
of replication initiation proteins
 
 
|-
 
|-
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 6 kDa, 5.605  
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 32 kDa, 5.187  
 
|-
 
|-
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|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 156 bp, 52 aa  
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|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 834 bp, 278 aa  
 
|-
 
|-
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yloV]]'', ''[[sdaAA]]''
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[bmr]]'', ''[[bkdB]]''
 
|-
 
|-
 
|colspan="2" style="background:#FAF8CC;" align="center"|'''Hier soll was neues rein'''
 
|colspan="2" style="background:#FAF8CC;" align="center"|'''Hier soll was neues rein'''
 
|-
 
|-
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|colspan="2" | '''Genetic context''' <br/> [[Image:sda_context.gif]]
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|colspan="2" | '''Genetic context''' <br/> [[Image:bmrR_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|-
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__TOC__
 
__TOC__
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<br/><br/>
 
<br/><br/>
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=The gene=
 
=The gene=
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===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
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*sporulates at a higher frequency
 
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*will sporulate in the presence of replication stress
 
  
 
=== Database entries ===
 
=== Database entries ===
  
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* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/sda.html]
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* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/bmrU-bmr-bmrR.html]
  
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* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG14183]
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* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10304]
  
 
=== Additional information===
 
=== Additional information===
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* '''Swiss prot entry:'''
 
* '''Swiss prot entry:'''
  
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* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU25690]
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* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU24020]
  
 
* '''E.C. number:'''
 
* '''E.C. number:'''
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=Expression and regulation=
 
=Expression and regulation=
  
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* '''Operon:'''  ''sda''
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* '''Operon:'''  
  
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* '''Sigma factor:''' [[SigA]]
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* '''[[Sigma factor]]:''' [[SigB]] [http://www.ncbi.nlm.nih.gov/pubmed/11544224 PubMed]  
  
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* '''Regulation:'''  [[DnaA]]
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* '''Regulation:'''  induced by stress ([[SigB]]) [http://www.ncbi.nlm.nih.gov/pubmed/11544224 PubMed]
  
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* '''Regulatory mechanism:''' Probably activated by [[DnaA]] under replication stress
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* '''Regulatory mechanism:'''  
  
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* '''Additional information:'''
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* '''Additional information:'''  
  
 
=Biological materials =
 
=Biological materials =
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=Labs working on this gene/protein=
 
=Labs working on this gene/protein=
 +
 +
[[Richard Brennan]], Houston, Texas, USA [http://www.mdanderson.org/departments/biochem/display.cfm?id=556ef368-6c81-4043-b74f350d41dd06cb&method=displayfull&pn=a8427ebd-d0ff-11d4-80fd00508b603a14 Homepage]
  
 
=Your additional remarks=
 
=Your additional remarks=
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=References=
 
=References=
  
 +
# Petersohn et al. (2001) Global Analysis of the General Stress Response of ''Bacillus subtilis''. ''J Bacteriol.'' '''183:''' 5617-5631 [http://www.ncbi.nlm.nih.gov/pubmed/11544224 PubMed]
 
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]
 
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]

Revision as of 15:48, 15 April 2009

  • Description: general stress protein, transcriptional activator of the bmrU-bmrR operon

Gene name bmrR
Synonyms
Essential no
Product transcriptional activator
Function regulation of multidrug resistance
MW, pI 32 kDa, 5.187
Gene length, protein length 834 bp, 278 aa
Immediate neighbours bmr, bkdB
Hier soll was neues rein
Genetic context
BmrR context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • Swiss prot entry:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Richard Brennan, Houston, Texas, USA Homepage

Your additional remarks

References

  1. Petersohn et al. (2001) Global Analysis of the General Stress Response of Bacillus subtilis. J Bacteriol. 183: 5617-5631 PubMed
  2. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed
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