Difference between revisions of "Sandbox"

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* '''Description:''' multidrug-efflux transporter (puromycin, nerfloxacin, tosufloxacin) <br/><br/>
+
* '''Description:''' lactate permease, excretion <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''mdr''
+
|''lctP''
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ycgD, bmr3 ''
+
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ycgC ''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || multidrug-efflux transporter (puromycin, nerfloxacin, tosufloxacin)
+
|style="background:#ABCDEF;" align="center"| '''Product''' || L-lactate permease
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || drug resistance
+
|style="background:#ABCDEF;" align="center"|'''Function''' || lactate excretion
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 46 kDa, 9.701  
+
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 57 kDa, 9.766  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1278 bp, 426 aa  
+
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1623 bp, 541 aa  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[lctP]]'', ''[[ycgE]]''
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ldh]]'', ''[[mdr]]''
 
|-
 
|-
 
|colspan="2" style="background:#FAF8CC;" align="center"|'''Hier soll was neues rein'''
 
|colspan="2" style="background:#FAF8CC;" align="center"|'''Hier soll was neues rein'''
Line 24: Line 24:
 
|-
 
|-
 
|-
 
|-
|colspan="2" | '''Genetic context''' <br/> [[Image:mdr_context.gif]]
+
|colspan="2" | '''Genetic context''' <br/> [[Image:lctP_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|-
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__TOC__
 
__TOC__
  
<br/><br/>
+
<br/><br/><br/><br/>
  
 
=The gene=
 
=The gene=
Line 45: Line 45:
 
* '''DBTBS entry:''' no entry
 
* '''DBTBS entry:''' no entry
  
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12002]
+
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12001]
  
 
=== Additional information===
 
=== Additional information===
Line 58: Line 58:
 
* '''Protein family:'''
 
* '''Protein family:'''
  
* '''Paralogous protein(s):'''
+
* '''Paralogous protein(s):''' [[LutP]]
  
 
=== Extended information on the protein ===
 
=== Extended information on the protein ===
Line 82: Line 82:
 
* '''Swiss prot entry:'''
 
* '''Swiss prot entry:'''
  
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU03070]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU03060]
  
 
* '''E.C. number:'''
 
* '''E.C. number:'''
Line 90: Line 90:
 
=Expression and regulation=
 
=Expression and regulation=
  
* '''Operon:'''  
+
* '''Operon:''' ''[[ldh]]-[[lctP]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/16207915 PubMed]
  
 
* '''[[Sigma factor]]:'''  
 
* '''[[Sigma factor]]:'''  
  
* '''Regulation:''' expression activated by glucose (3.6 fold) [http://www.ncbi.nlm.nih.gov/pubmed/12850135 PubMed]
+
* '''Regulation:''' induced under anaerobic conditions ([[Rex]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/16207915 PubMed]
  
* '''Regulatory mechanism:'''  
+
* '''Regulatory mechanism:''' [[Rex]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/16207915 PubMed]
  
 
* '''Additional information:'''
 
* '''Additional information:'''
Line 120: Line 120:
 
=References=
 
=References=
  
# Blencke et al. (2003) Transcriptional profiling of gene expression in response to glucose in ''Bacillus subtilis'': regulation of the central metabolic pathways. ''Metab Eng.'' '''5:''' 133-149 [http://www.ncbi.nlm.nih.gov/pubmed/12850135 PubMed]
 
 
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]
 
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]

Revision as of 15:42, 15 April 2009

  • Description: lactate permease, excretion

Gene name lctP
Synonyms ycgC
Essential no
Product L-lactate permease
Function lactate excretion
MW, pI 57 kDa, 9.766
Gene length, protein length 1623 bp, 541 aa
Immediate neighbours ldh, mdr
Hier soll was neues rein
Genetic context
LctP context.gif
This image was kindly provided by SubtiList





The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s): LutP

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • Swiss prot entry:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation: induced under anaerobic conditions (Rex) PubMed
  • Regulatory mechanism: Rex: transcription repression PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed