Difference between revisions of "Sandbox"

From SubtiWiki
Jump to: navigation, search
Line 1: Line 1:
* '''Description:''' phospho-N-acetylmuramoyl-pentapeptide-transferase (meso-2,6-diaminopimelate) <br/><br/>
+
* '''Description:''' mother-cell specific penicillin-binding protein (spore cortex) <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''mraY''
+
|''spoVD''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
 
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
+
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || phospho-N-acetylmuramoyl-pentapeptide-transferase (meso-2,6-diaminopimelate)
+
|style="background:#ABCDEF;" align="center"| '''Product''' || penicillin-binding protein (spore cortex)
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || peptidoglycan precursor biosynthesis
+
|style="background:#ABCDEF;" align="center"|'''Function''' || spore morphogenesis
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 35 kDa, 8.966  
+
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 71 kDa, 8.85  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 972 bp, 324 aa  
+
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1935 bp, 645 aa  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[murE]]'', ''[[murD]]''
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[pbpB]]'', ''[[murE]]''
 
|-
 
|-
 
|colspan="2" style="background:#FAF8CC;" align="center"|'''Hier soll was neues rein'''
 
|colspan="2" style="background:#FAF8CC;" align="center"|'''Hier soll was neues rein'''
 
|-
 
|-
|colspan="2" | '''Genetic context''' <br/> [[Image:mraY_context.gif]]
+
|colspan="2" | '''Genetic context''' <br/> [[Image:spoVD_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|-
Line 38: Line 38:
  
 
===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
 
essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
 
  
 
=== Database entries ===
 
=== Database entries ===
  
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/murE-mraY-murD-spoVE-murG-murB-divIB-ylxWX-sbp.html]
+
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/spoVD.html]
  
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10224]
+
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10222]
  
 
=== Additional information===
 
=== Additional information===
Line 82: Line 80:
 
* '''Swiss prot entry:'''
 
* '''Swiss prot entry:'''
  
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU15190]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU15170]
  
 
* '''E.C. number:'''
 
* '''E.C. number:'''
Line 90: Line 88:
 
=Expression and regulation=
 
=Expression and regulation=
  
* '''Operon:''' [[murE]]-[[mraY]]-[[murD]]-[[spoVE]]-[[murG]]-[[murB]]-[[divIB]]-[[ylxW]]-[[ylxX]]-[[sbp]]
+
* '''Operon:'''  
  
* '''[[Sigma factor]]:''' [[SigE]]
+
* '''[[Sigma factor]]:''' [[SigE]] [http://www.ncbi.nlm.nih.gov/sites/entrez/15758244 Link to a summary]
  
* '''Regulation:'''  
+
* '''Regulation:''' expressed during sporulation in the mother cell [http://www.ncbi.nlm.nih.gov/sites/entrez/15758244 Link to a summary]
  
 
* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
  
* '''Additional information:'''
+
* '''Additional information:'''  
  
 
=Biological materials =
 
=Biological materials =
Line 120: Line 118:
 
=References=
 
=References=
  
# Al-Dabbagh B, Henry X, El Ghachi M, Auger G, Blanot D, Parquet C, Mengin-Lecreulx D, Bouhss A. (2008) Active site mapping of MraY, a member of the polyprenyl-phosphate N-acetylhexosamine 1-phosphate transferase superfamily, catalyzing the first membrane step of peptidoglycan biosynthesis. ''Biochemistry.'' '''Aug 26;47(34):''' 8919-28.  [http://www.ncbi.nlm.nih.gov/sites/entrez/18672909 PubMed]
 
 
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]
 
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]

Revision as of 15:25, 15 April 2009

  • Description: mother-cell specific penicillin-binding protein (spore cortex)

Gene name spoVD
Synonyms
Essential no
Product penicillin-binding protein (spore cortex)
Function spore morphogenesis
MW, pI 71 kDa, 8.85
Gene length, protein length 1935 bp, 645 aa
Immediate neighbours pbpB, murE
Hier soll was neues rein
Genetic context
SpoVD context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • Swiss prot entry:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed