Difference between revisions of "RocG"

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Gene rocG
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* '''Description:''' write here <br/><br/>
  
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Codes for RocG, glutamate dehydrogenase
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{| align="right" border="1" cellpadding="2"
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|-
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|style="background:#ABCDEF;" align="center"|'''Gene name'''
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|''genE''
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|-
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|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
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|-
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|style="background:#ABCDEF;" align="center"| '''Essential''' || write here
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|-
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|style="background:#ABCDEF;" align="center"| '''Product''' || write here
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|-
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|style="background:#ABCDEF;" align="center"|'''Function''' || write here
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|-
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || xx,x kDa, y.yy
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|-
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|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || aaa bp, bbb amino acids
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|-
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[genA]]'', ''[[genB]]''
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|-
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|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/genE_nucleotide.txt    Gene sequence      (+200bp)  ]'''
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|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/genE_protein.txt Protein sequence]'''
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|-
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|colspan="2" | '''Genetic context''' <br/> [[Image:genE_context.gif]]
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|-
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|}
  
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=Basic information=
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__TOC__
  
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Synonyms
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<br/><br/><br/><br/><br/><br/><br/><br/><br/><br/>
  
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Coordinates
 
  
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DNA sequence
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=The gene=
  
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Protein sequence
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=== Basic information ===
  
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Essential N
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* '''Coordinates:'''
  
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MW
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===Phenotypes of a mutant ===
  
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pI
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=== Database entries ===
  
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=Basic information on the protein=
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* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/rocG.html]
  
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Catalyzed reaction/ biological activity
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* '''SubtiList entry:'''
  
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Protein family
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=== Additional information===
  
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Structure
 
  
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Swiss prot entry
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=The protein=
  
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KEGG entry
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=== Basic information/ Evolution ===
  
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Extended information on the protein
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* '''Catalyzed reaction/ biological activity:'''
  
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Kinetic information
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* '''Protein family:'''
  
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Domains
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* '''Paralogous protein(s):'''
  
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Modification
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=== Extended information on the protein ===
  
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Effectors of protein activity
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* '''Kinetic information:'''
  
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IAX RocG-GltC
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* '''Domains:'''
  
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Localization
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* '''Modification:'''
  
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* '''Cofactor(s):'''
  
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Expression
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* '''Effectors of protein activity:'''
  
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Operon rocG
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* '''Interactions:'''
  
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Sigma factor SigL
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* '''Localization:'''
  
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Regulation induced by arginine (RocR-dependent)
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=== Database entries ===
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Repressed by glucose (CcpA-dependent)
 
  
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* '''Structure:'''
  
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Regulatory mechanism RocR: transcription activation, involves long-range DNA looping
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* '''Swiss prot entry:'''
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CcpA: transcription repression
 
  
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* '''KEGG entry:'''
  
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Biological Materials
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* '''E.C. number:'''
  
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Mutant GP747, spc (Stülke lab)
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=== Additional information===
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GP726, aphA3 (Stülke lab)
 
  
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Expression vector pGP259 (Expression in B. subtilis, based on pBQ200)
 
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pGP902 (Strep-tag, based on pGP172)
 
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pGP906 (Strep-tag, Expression in B. subtilis, based on pBQ200)
 
  
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lacZ fusion
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=Expression and regulation=
  
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GFP fusion
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* '''Operon:'''
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Antibody Stülke lab
 
  
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* '''Sigma factor:'''
  
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* '''Regulation:'''
  
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Phenotypes of a Mutant
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* '''Regulatory mechanism:'''
  
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* '''Additional information:'''
  
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=Biological materials =
  
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homologous gene(s) in B. subtilis gudB (cryptic glutamate dehydrogenase)
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=Labs working on this gene/protein=
  
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additional remarks
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=Your additional remarks=
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=References=
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# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]

Revision as of 15:45, 17 December 2008

  • Description: write here

Gene name genE
Synonyms
Essential write here
Product write here
Function write here
MW, pI xx,x kDa, y.yy
Gene length, protein length aaa bp, bbb amino acids
Immediate neighbours genA, genB
Gene sequence (+200bp) Protein sequence
Genetic context
File:GenE context.gif












The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry:

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • Swiss prot entry:
  • KEGG entry:
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

Labs working on this gene/protein

Your additional remarks

References

  1. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed
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