scpB

scpB
168

part of the condensin complex, chromosomal origin condensation and segregation

Locus
BSU_23210
Molecular weight
21.89 kDa
Isoelectric point
4.25
Protein length
197 aaSequenceBlast
Gene length
594 bpSequenceBlast
Function
segregation of replication origins
Product
DNA segregation and condensation protein
Essential
no
Synonyms
scpB, ypuH, spcB
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1386 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,425,248  2,425,841
Phenotypes of a mutant
essential PubMed, non-essential according to PubMed
''scpB'' mutants are not viable on complex medim that allow rapid growth, but they are viable under conditions of slow growth PubMed
The protein
Catalyzed reaction/ biological activity
negatively regulates binding of the ScpA NTD to the Smc neck region PubMed
Protein family
scpB family (single member, according to UniProt)
Structure
4I98 (PDB) (complex between ScpA (aa 1-160)-ScpB (aa 1-183) PubMed
P35155 (AlphaFold)
Localization
nucleoid (Multiple) PubMed
the condensin (Smc)2-ScpA-ScpB complex is loaded by ParB to parS centromeric sites adjacent to the replication origin, travels then from the origin to the terminus at rates >50 kb per minute PubMed
Expression and Regulation
Operons
Genes
scpA-scpB-ypuI
Description
PubMed
Regulation
RelA dependent downregulation (Class I) during stringent response PubMed
Regulatory mechanism
stringent response: negative regulation, in stringent response
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scpA→ypuI

2025-04-08 02:19:55

ghost

134

962f5d9cd284750c7b8d6dafae170ff7097452f9

E653E2EC69211554D00603C6268E7EDE01F77C4F

Biological materials
Mutant
MGNA-A105 (ypuH::erm), available at the NBRP B. subtilis, Japan
BKE23210 (scpB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACAATAGCTTTCCAATTCA,  downstream forward: _UP4_TAGAAGTTTAATGAATCGCG
BKK23210 (scpB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACAATAGCTTTCCAATTCA,  downstream forward: _UP4_TAGAAGTTTAATGAATCGCG
References
Reviews
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Original Publications
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9137D162E6331E2B127472BCF5F49A9AE46266F3

Page visits: 4045

Time of last update: 2025-04-11 11:03:38

Author of last update: Jstuelk