spoIISA

spoIISA
168

lethal when synthesized during vegetative growth in the absence of SpoIISB

Locus
BSU_12830
Molecular weight
28.91 kDa
Isoelectric point
4.68
Protein length
248 aaSequenceBlast
Gene length
747 bpSequenceBlast
Function
programmed cell death
Product
toxin
Essential
no
Synonyms
spoIISA, ykaC
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms

This gene is a member of the following regulons

Gene
Coordinates
1,348,612 → 1,349,358
The protein
Protein family
SpoIISA toxin family (single member, according to UniProt)
Domains
three putative membrane-spanning segments and a cytoplasmic domain
Structure
3O6Q (PDB) (cytoplasmic fragment of SpoIISA (CSpoIISA) in complex with SpoIISB) PubMed
O34853 (AlphaFold)
Localization
cell membrane PubMed
Expression and Regulation
Operons
Genes
spoIISA-spoIISB
Description
PubMed
Sigma factors
SigK: sigma factor, in sigK regulon
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spoIISA→spoIISB

2025-04-07 08:18:05

ghost

129

dba0540459879cc4ec595c8048a437eb0a18061d

5789610C653201DDB59376F461D88F12DF191D72

Biological materials
Mutant
MGNA-A006 (spoIISA::erm), available at the NBRP B. subtilis, Japan
1S123 ( spoIISA::kan), PubMed, available at BGSC
BKE12830 (ΔspoIISA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATTCGAATCCCTCACAT,  downstream forward: _UP4_ATTGAGGAGGAAGGTGAAGG
BKK12830 (ΔspoIISA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATTCGAATCCCTCACAT,  downstream forward: _UP4_ATTGAGGAGGAAGGTGAAGG
References
Reviews
Brantl S, Müller P Toxin⁻Antitoxin Systems in . Toxins. 2019 May 09; 11(5). pii:E262. doi:10.3390/toxins11050262. PMID:31075979
Original Publications
Gabriško M, Barák I Evolution of the SpoIISABC Toxin-Antitoxin-Antitoxin System in Bacilli. Toxins. 2016 Jun 09; 8(6). doi:10.3390/toxins8060180. pii:E180. PMID:27294956
Melničáková J, Bečárová Z, Makroczyová J, Barák I Analysis of the Bacillus cereus SpoIIS antitoxin-toxin system reveals its three-component nature. Frontiers in microbiology. 2015; 6:808. doi:10.3389/fmicb.2015.00808. PMID:26300872
Makroczyová J, Rešetárová S, Florek P, Barák I Topology of the Bacillus subtilis SpoIISA protein and its role in toxin-antitoxin function. FEMS microbiology letters. 2014 Sep; 358(2):180-7. doi:10.1111/1574-6968.12531. PMID:25039482
Florek P, Levdikov VM, Blagova E, Lebedev AA, Škrabana R, Resetárová S, Pavelcíková P, Barak I, Wilkinson AJ The structure and interactions of SpoIISA and SpoIISB, a toxin-antitoxin system in Bacillus subtilis. The Journal of biological chemistry. 2011 Feb 25; 286(8):6808-19. doi:10.1074/jbc.M110.172429. PMID:21147767
Rešetárová S, Florek P, Muchová K, Wilkinson AJ, Barák I Expression and localization of SpoIISA toxin during the life cycle of Bacillus subtilis. Research in microbiology. 2010 Nov; 161(9):750-6. doi:10.1016/j.resmic.2010.09.005. PMID:20863891
Florek P, Muchová K, Pavelcíková P, Barák I Expression of functional Bacillus SpoIISAB toxin-antitoxin modules in Escherichia coli. FEMS microbiology letters. 2008 Jan; 278(2):177-84. . PMID:18096016
Adler E, Barák I, Stragier P Bacillus subtilis locus encoding a killer protein and its antidote. Journal of bacteriology. 2001 Jun; 183(12):3574-81. . PMID:11371520

CD14C88E9976964A0D25BAA22C395A5A6951654D

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Time of last update: 2025-04-11 23:05:47

Author of last update: Melvin.boenninger