salA

salA
168

negative regulator of scoC expression, activator of PtkA kinase activity, similar to Fe-S cluster carrier protein

Locus
BSU_01540
Molecular weight
38.48 kDa
Isoelectric point
5.23
Protein length
352 aaSequenceBlast
Gene length
1059 bpSequenceBlast
Function
control of alkaline protease expression
Product
MRP family regulator
Essential
no
Synonyms
salA, ybaL
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0489 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
157,421  158,479
Phenotypes of a mutant
increased expression of ''aprE (due to loss of repression of scoC'' expression)
The protein
Catalyzed reaction/ biological activity
transcription repression of ''scoC'' expression 26094643
Protein family
Mrp/NBP35 ATP-binding proteins family (single member, according to UniProt)
Domains
DNA-binding domain: aa 1 - 60 PubMed
ATP-binding Walker domain: aa 105 - 275 PubMed
domain for the interaction with PtkA: aa 294 - 352
Structure
8ZKC (PDB) (from E. coli, 32.8% identity) PubMed
4V03 (PDB) (MinD from Aquifex aeolicus, corresponds to C-terminal domain, aa 109 ... 344, 28% identity) PubMed
P50863 (AlphaFold)
Modification
PtkA-dependent phosphorylation at Tyr-327 results in better binding of the target promoter PubMed
Expression and Regulation
Operons
Genes
salA
Description
PubMed
Regulatory mechanism
SalA: repression, in salA regulon
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salA

2025-03-18 21:13:09

Bzhu

73

08202f127813ccabafb58b39507cc5796a50a4b9

906C5D4C503D12520525B152C613A2DDE709A5EC

Biological materials
Mutant
1A919 ( salA::erm), PubMed, available at BGSC
BKE01540 (salA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCGAACCTCACCCTTTTG,  downstream forward: _UP4_TAAAAGGTGAACCGGGATTC
BKK01540 (salA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCGAACCTCACCCTTTTG,  downstream forward: _UP4_TAAAAGGTGAACCGGGATTC
References
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8841E35B7A90F6716C0BCD98E8D0CC05FE1CA471

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Time of last update: 2025-04-07 03:40:03

Author of last update: Jstuelk