ptsH
168

HPr, General component of the sugar phosphotransferase system (PTS)

Locus

BSU_13900

Molecular weight

9.05 kDa

Isoelectric point

4.58

Protein length

88 aaSequenceBlast

Gene length

267 bpSequenceBlast

Function

PTS-dependent sugar transport and carbon catabolite repression

Product

histidine-containing phosphocarrier protein HPr of the PTS

Essential

E.C.

2.7.11.-

Synonyms

ptsH, HPr

Outlinks

BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

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Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1925 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene

Coordinates

1,459,384 1,459,650

Phenotypes of a mutant

the mutant cells are thinner and shorter than wild type cells PubMed

The protein

Protein family

HPr family (with Crh, according to UniProt)

Domains

HPr domain (aa 2-88) (according to UniProt)

Structure

2FEP (PDB) PubMed

2HID (PDB) (NMR) PubMed

1KKM (PDB) (complex of L. casei HprK with B. subtilis HPr-Ser-P)

1KKL (PDB) (complex of Lactobacillus casei HprK with B. subtilis HPr)

3OQM (PDB) (complex of B. subtilis CcpA with P-Ser-HPr and the ackA operator site)

3OQN (PDB) (complex of B. subtilis CcpA with P-Ser-HPr and the gntR operator site)

3OQO (PDB) (complex of B. subtilis CcpA with P-Ser-HPr and a optimal synthetic operator site)

P08877 (AlphaFold)

Modification

transient phosphorylation by Enzyme I of the PTS on His-15

regulatory phosphorylation on Ser-46 by HprK PubMed

an extensive study on in vivo HPr phosphorylation can be found in Singh et al. (2008) PubMed

weak phosphorylation on Ser-12 PubMed

in vitro phosphorylated by PrkC on Ser-12 PubMed

Paralogous protein(s)

Crh

Additional information

belongs to the 100 most abundant proteins PubMed

Expression and Regulation

Operons

Genes

ptsH-ptsI

Description

PubMed

Sigma factors

SigA: sigma factor, PubMed, in sigA regulon

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Genes

ptsG-ptsH-ptsI

Description

PubMed

Regulation

expression activated by glucose (2 fold) (GlcT) PubMed

Regulatory mechanism

stringent response: negative regulation, in stringent response

GlcT: antitermination, via the GlcT-dependent RNA switch PubMed, in glcT regulon

Sigma factors

SigA: sigma factor, PubMed, in sigA regulon

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Biological materials

Mutant

available in Jörg Stülke's lab:

MZ303 (cat)

GP507 ptsH1 (S46A)

GP506 (ptsH-H15A)

GP778 (glcT-ptsG-ptsH-ptsI::spc) PubMed

BKE13900 (ptsH::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAAGAATTGACCTCCTCT, downstream forward: _UP4_TAAGGGTGTTAGTACGCCGT

BKK13900 (ptsH::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAAGAATTGACCTCCTCT, downstream forward: _UP4_TAAGGGTGTTAGTACGCCGT

Expression vectors

pGP438 (with N-terminal Strep-tag, in pGP172), available in Jörg Stülke's lab

pAG2 (His-tag) PubMed, available in Anne Galinier lab

pGP371(expression / purification of HPr-S46A, with His-tag from E. coli, in pWH844), available in Jrg Stlke's lab

pGP1415 (HPr, expression in B. subtilis, from pBQ200), available in Jörg Stülke's lab

pGP961 (HPr, expression in B. subtilis with N-terminal Strep-tag, for SPINE, available in Jörg Stülke's lab

pGP1416 (HPr-H15A, expression in B. subtilis, from pBQ200), available in Jörg Stülke's lab

pGP3628 expression of Strep-ptsH by pGP380 in B. subtilis suitable for SPINE, available in Jörg Stülke's lab

Two-hybrid system

B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab

Antibody

available in Jörg Stülke's lab

GFP fusion

GP1267, ptsH-cfp, available in Jörg Stülke's lab PubMed

Labs working on this gene/protein

Jörg Stülke, University of Gttingen, Germany Homepage

Richard Brennan, Houston, Texas, USA Homepage

Anne Galinier, University of Marseille, France

References

Reviews

Original Publications

Page visits: 15467

Time of last update: 2025-04-04 14:20:49

Author of last update: Jstuelk

ptsH 168

ptsH
168