murG
168

UDP-N-acetylglucosamine-N-acetylmuramyl-(pentapeptide)pyrophosphoryl-undecaprenol N-acetylglucosamine transferase, conversion of lipid I to lipid II

Locus

BSU_15220

Molecular weight

39.82 kDa

Isoelectric point

9.57

Protein length

363 aaSequenceBlast

Gene length

1092 bpSequenceBlast

Function

peptidoglycan precursor biosynthesis

Product

UDP-N-acetylglucosamine-N-acetylmuramyl-(pentapeptide)pyrophosphoryl-undecaprenol N-acetylglucosamine transferase

Essential

yes

E.C.

2.4.1.227

Synonyms

murG

Outlinks

BsubCyc SubtiList UniProt STRING Genoscapist

Genomic Context

Hide small RNAsZoom:

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0707 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene

Coordinates

1,591,540 → 1,592,631

Phenotypes of a mutant

essential PubMed

The protein

Catalyzed reaction/ biological activity

converts lipid I to lipid II by attaching GlcNAc to the bactoprenol carrier-conjugated UDP-N-acetylmuramic acid (UDP-MurNAc) pentapeptide molecule

Mur2Ac(oyl-L-Ala-γ-D-Glu-L-Lys-D-Ala-D-Ala)-diphospho-di-trans,octa-cis-undecaprenol + UDP-N-acetyl-α-D-glucosamine --> β-D-GlcNAc-(1→4)-Mur2Ac(oyl-L-Ala-γ-D-Glu-L-Lys-D-Ala-D-Ala)-diphospho-di-trans,octa-cis-undecaprenol + H⁺ + UDP (according to UniProt)

Protein family

glycosyltransferase 28 family (with UgtP and YkoN, according to UniProt)

Structure

1F0K (PDB) (from Escherichia coli, 30% identity, 52% similarity) PubMed

P37585 (AlphaFold)

Effectors of protein activity

activity during sporulation is stimulated by SpoVK PubMed

Localization

peripheral membrane protein PubMed

associates with membrane areas of increased fluidity PubMed

in the presence of daptomycin, MurG delocalizes from the membrane to the cytoplasm PubMed

forms banded patterns along the length of the cell, this pattern formation depends on MreB PubMed