radA

radA
168

branch migration transferase, 6-O-methylguanine-DNA methyltransferase, negative effector of DisA activity, participates in the stabilization and/or processing of holliday junction intermediates, required for repair-by-recombination, crucial for the RecA-dependent acquisition of homologous genes from related species by natural transformation

Locus
BSU_00870
Molecular weight
49.32 kDa
Isoelectric point
8.13
Protein length
458 aaSequenceBlast
Gene length
1377 bpSequenceBlast
Function
homologous recombination, control of c-di-AMP formation, co-ordination of responses to replicative stress and genetic recombination
Product
branch migration transferase, 6-O-methylguanine-DNA methyltransferase
Essential
no
Synonyms
radA, sms, yacJ
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1066 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
106,096  107,472
Phenotypes of a mutant
reduced spore survival after infrared exposure PubMed
sensitive to mitomycin C and H2O2-induced DNA damage PubMed
reduced formation of rpsB or rpsE suppressor mutants after mitomycin treatment PubMed
impaired in transformation with chromosomal DNA PubMed
no transformation with chromosomal DNA in recG mutant cells PubMed
The protein
Catalyzed reaction/ biological activity
reduces the activity of DisA PubMed
binds ssDNA and Holliday junctions (preferentially over dsDNA) PubMed
promotes RecA-mediated DNA strand exchange PubMed
ATPase and 5' --> 3' DNA helicase activity PubMed
unwinds forked DNA in the 5′→3′ direction PubMed
Protein family
RecA family (together with RecA) (according to UniProt)
Domains
N-terminal zinc finger (aa 10 ... 27) (according to UniProt)
central RecA-like ATPase domain (according to UniProt)
KNRFG motif (aa 255 ... 259) (according to UniProt)
C-terminal Lon domain (aa 354 ... 458) (according to UniProt)
Structure
5LKM (PDB) (from Streptococcus pneumoniae, 63% identity) PubMed
P37572 (AlphaFold)
Localization
cytoplasm (according to UniProt)
Expression and Regulation
Operons
Genes
ctsR-mcsA-mcsB-clpC-radA-disA
Description
PubMed
Regulation
expressed during germination and spore outgrowth PubMed
Regulatory mechanism
CtsR: repression, PubMed, in ctsR regulon
Spx: activation, PubMed, in spx regulon
Sigma factors
SigM: sigma factor, PubMed, in sigM regulon
SigA: sigma factor, PubMed, in sigA regulon
SigB: sigma factor, PubMed PubMed, in sigB regulon
SigF: sigma factor, PubMed, in sigF regulon
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ctsR→disA

2025-04-02 20:37:12

Bzhu

159

b7066b5ca5004b095502d71420c469e9ee925bfa

163769A1708F45F8ABE642374A97FA8A00293529

Biological materials
Mutant
MGNA-B931 (yacJ::erm), available at the NBRP B. subtilis, Japan
BKG1 (''radA::spc''), available in Jörg Stülke's lab
BKG2 (''radA-disA::spc''), available  in Jörg Stülke's lab
BKE00870 (radA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATATAGTGTAAGACCTC,  downstream forward: _UP4_CGTACTTCATTAGGAGGATA
BKK00870 (radA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATATAGTGTAAGACCTC,  downstream forward: _UP4_CGTACTTCATTAGGAGGATA
Expression vectors
pGP2693: expression in E. coli, with C-terminal Strep-tag, in pGP574, available in Jörg Stülke's lab
References
Reviews
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Original Publications
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151F226370D225776F3FE7EA4901485095F1AC45

Page visits: 5257

Time of last update: 2025-04-07 07:36:12

Author of last update: Jstuelk