ruvA

ruvA
168

Holliday junction DNA helicase, part of the RuvA-RuvB branch migration translocase and the RuvA-RuvB-RecU resolvasome

Locus
BSU_27740
Molecular weight
22.44 kDa
Isoelectric point
5.38
Protein length
201 aaSequenceBlast
Gene length
606 bpSequenceBlast
Function
DNA repair/ recombination
Product
Holliday junction DNA helicase
Essential
no
Synonyms
ruvA
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0632 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,836,165  2,836,770
Phenotypes of a mutant
a ruvA-ruvB recG mutant is not viable PubMed
the inactivations of ''recU and ruvA-ruvB'' are synthetically lethal PubMed
the inactivations of ''recD2 and ruvA-ruvB'' are synthetically lethal  PubMed
The protein
Catalyzed reaction/ biological activity
inhibits resolution of Holliday junctions by RecU-RuvB PubMed
ATP + H2O --> ADP + H+ + phosphate (according to UniProt)
Protein family
ruvA family (single member, according to UniProt)
Structure
1BDX (PDB) (from E. coli, 34% identity) PubMed
O05392 (AlphaFold)
Effectors of protein activity
DisA PubMed
Interactions
part of the RuvA-RuvB-RecU resolvasome PubMed
Expression and Regulation
Operons
Genes
ruvA-ruvB-yrzS-queA-tgt-yrbF
Description
PubMed
Regulation
the mRNA is processed between tgt and yrbF by RNase Y, this requires the RicA-RicF-RicT complex PubMed
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ruvA→yrbF

2025-03-27 05:06:27

Jstuelk

112

b67b31b8062f65eb2771aa03f56aa1b45473760c

1495BBE3D6ACD7DA206B13AA8930F5C46B2B7309

Genes
ruvA-ruvB
Description
PubMed
Regulation
induced upon DNA damage (LexA) PubMed
Regulatory mechanism
LexA: repression, PubMed, in lexA regulon
Open in new tab

ruvA→ruvB

2025-03-09 08:46:47

ghost

82

399d959e76cbadc6fc544f5c054f1a8ef04ab38e

D85734A1213FB25374867F58D9E67BF1C968590E

Biological materials
Mutant
GP3530 (Δ(ruvA-ruvB)::kan), available in Jörg Stülke's lab
1A896 (no resistance), PubMed, available at BGSC
BKE27740 (ruvA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACGCAAATTCACCTCTTTG,  downstream forward: _UP4_AAACTATTAAAGTAAGGTGA
BKK27740 (ruvA::kan  trpC2) available at BGSC and in Jörg Stülke's lab,  PubMed, upstream reverse: _UP1_CACGCAAATTCACCTCTTTG,  downstream forward: _UP4_AAACTATTAAAGTAAGGTGA
References
Reviews
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Original Publications
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7EBC90946E7167A3B0212193873855CAE0EFF7D4

Page visits: 3429

Time of last update: 2025-04-12 15:17:58

Author of last update: Jstuelk