leuB

leuB
168

3-isopropylmalate dehydrogenase

Locus
BSU_28270
Molecular weight
39.79 kDa
Isoelectric point
4.74
Protein length
365 aaSequenceBlast
Gene length
1098 bpSequenceBlast
Function
biosynthesis of leucine
Product
3-isopropylmalate dehydrogenase
Essential
no
E.C.
1.1.1.85
Synonyms
leuB, leuC
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0473 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,891,020 2,892,117
The protein
Catalyzed reaction/ biological activity
(2R,3S)-3-isopropylmalate + NAD+ --> 4-methyl-2-oxopentanoate + CO2 + NADH (according to UniProt)
Protein family
Isocitrate and isopropylmalate dehydrogenases family (with Icd and YcsA, according to UniProt)
Structure
1XAD (PDB) (Thermus thermophilus)
P05645 (AlphaFold)
Modification
phosphorylated on Arg-4 PubMed
Paralogous protein(s)
YcsA
Additional information
belongs to the 100 most abundant proteins PubMed
Expression and Regulation
Operons
Genes
ilvB-ilvH-ilvC-leuA-leuB-leuC-leuD
Description
PubMed
Regulation
expression is stimulated in the presence of glucose (CcpA) PubMed
Regulatory mechanism
T-box: RNA switch, via tRNA controlled RNA switch, repression by BCAA, in T-box
TnrA: repression, PubMed, in tnrA regulon
CodY: repression, PubMed PubMed, in codY regulon
CcpA: activation, PubMed, in ccpA regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
Additional information
An antisense RNA is predicted for leuA PubMed
the T-box RNA is degraded by RNase Y PubMed
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ilvB→leuD

2025-04-04 04:53:41

Jstuelk

148

905a0fc4b9e1945f65cf0820ea8bda45e4ac716c

931C13BE07545B8037A118810CF4C8E29210A7CE

Biological materials
Mutant
1A618 ( leuB::erm), PubMed, available at BGSC
BKE28270 (leuB::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAAGTTAACCGTTCTCCTTT, downstream forward: _UP4_TGACAGCTTACGTTAAGCGG
BKK28270 (leuB::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAAGTTAACCGTTCTCCTTT, downstream forward: _UP4_TGACAGCTTACGTTAAGCGG
References
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Page visits: 6056

Time of last update: 2025-04-11 20:34:45

Author of last update: Melvin.boenninger