gltB

gltB
168

small subunit of glutamate synthase

Locus
BSU_18440
Molecular weight
54.78 kDa
Isoelectric point
7.7
Protein length
493 aaSequenceBlast
Gene length
1482 bpSequenceBlast
Function
glutamate biosynthesis
Product
glutamate synthase (small subunit)
Essential
no
E.C.
1.4.1.13
Synonyms
gltB
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0493 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,008,572  2,010,053
Phenotypes of a mutant
auxotrophic for glutamate PubMed, can be bypassed by inactivation of citG and ansR (as a result of ansA-ansB derepression)PubMed
The protein
Catalyzed reaction/ biological activity
2 L-glutamate + NADP+ --> 2-oxoglutarate + H+ + L-glutamine + NADPH (according to UniProt)
Protein family
glutamate synthase family (with GltA and YerD, according to UniProt)
Domains
nucleotide binding domain (NADP) (299313)
Cofactors
two 4Fe-4S clusters reference
Structure
2VDC (PDB) (the GltA-GltB complex of Azospirillum brasiliense) PubMed, note that B. subtilis GltA is unable to form dimers, thus GltA-GltB exists as a heterodimer PubMed
7MFT (PDB) (the GudB6-(GltA-GltB)6 complex) PubMed
O34399 (AlphaFold)
Localization
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
Genes
gltA-gltB
Description
PubMed
Regulation
expressed in the presence of ammonium PubMed
Regulatory mechanism
TnrA: repression, PubMed, in tnrA regulon
GltC: activation, PubMed, in gltC regulon
Fur: indirect effect, in fur regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
Open in new tab

gltA→gltB

2025-04-05 06:00:24

Bzhu

137

90b560615ccdd0da363a7f12dfb0b2af980e6000

F7C02591D79E87C32CC816954DB16451EB2276C9

Other regulations
FsrA: translation inhibition
Biological materials
Mutant
BP123 (ΔgltA-gltB::ermC) , available in Fabian Commichau's lab
GP807 (ΔgltA-gltB::tet) , available in Jörg Stülke's lab
GP517 (ΔgltB::ermC), available in Jörg Stülke's lab
BKE18440 (gltB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTTCCTCTCCCCTTCCT,  downstream forward: _UP4_TAAATAAAGGGGATTATCAT
BKK18440 (gltB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTTCCTCTCCCCTTCCT,  downstream forward: _UP4_TAAATAAAGGGGATTATCAT
Expression vectors
pGP1119 (in pGP380, for SPINE, expression in B. subtilis), available in Jörg Stülke's lab PubMed
pGP3031: IPTG inducible expression, purification in E. coli with C-terminal His-tag, in pGP574 (Strep-tag omitted), available in Jörg Stülke's lab
pGP3032: IPTG inducible expression, purification in E. coli with C-terminal Strep-tag, in pGP574, available in Jörg Stülke's lab
Two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
LacZ fusion
see ''gltA
Labs working on this gene/protein
Linc Sonenshein, Tufts University, Boston, MA, USA Homepage
Fabian Commichau, Göttingen, Germany homepage
References
Reviews
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Original Publications
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AA07CC52B2DD48ACC9D3375E9D531CB1ACBE485A

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Time of last update: 2025-04-06 18:05:48

Author of last update: Jstuelk