ansZ

ansZ
168

L-asparaginase

Locus
BSU_02690
Molecular weight
39.94 kDa
Isoelectric point
6.15
Protein length
375 aaSequenceBlast
Gene length
1128 bpSequenceBlast
Function
utilization of asparagine
Product
L-asparaginase
Essential
no
E.C.
3.5.1.1
Synonyms
ansZ, yccC
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0252 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
290,915 → 292,042
The protein
Catalyzed reaction/ biological activity
H2O + L-asparagine --> L-aspartate + NH4+(according to UniProt)
Protein family
asparaginase 1 family (with AnsA, according to UniProt)
Domains
Asparaginase/glutaminase domain (aa 51-375) (according to UniProt)
Structure
1ZCF (PDB) (from Pectobacterium atrosepticum scri1043, 55% identity, 73% similarity)
O34482 (AlphaFold)
Paralogous protein(s)
AnsA
Expression and Regulation
Operons
Genes
ansZ
Description
PubMed
Regulation
expressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed
Regulatory mechanism
TnrA: activation, PubMed, in tnrA regulon
CodY: repression, PubMed, in codY regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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ansZ

2025-03-20 19:07:20

ghost

117

7ba787f9d58f1c43788b137c41024d02e5e4783e

403FC861CA5313C809F7F9CA4914371DA0229410

Biological materials
Mutant
GP4247 (ΔansZ::kan), available in Jörg Stülke's lab
GP4248 (ΔansZ::cat), available in Jörg Stülke's lab
BKE02690 (ΔansZ::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTATTTCTCTCCTTACA,  downstream forward: _UP4_TGAAGAAAAGAAGGCGAATA
BKK02690 (ΔansZ::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTATTTCTCTCCTTACA,  downstream forward: _UP4_TGAAGAAAAGAAGGCGAATA
References
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Page visits: 7369

Time of last update: 2025-04-04 11:13:51

Author of last update: Jstuelk