pxpB

pxpB
168

subunit of ATP-dependent 5-oxoprolinase, inhibitor of KinA, control of the phosphorelay

Locus
BSU_04080
Molecular weight
26.57 kDa
Isoelectric point
4.66
Protein length
240 aaSequenceBlast
Gene length
723 bpSequenceBlast
Function
detoxification of 5-oxoproline, control of the phosphorelay, initiation of sporulation
Product
subunit of 5-oxoprolinase, inhibitor of KinA
Essential
no
Synonyms
pxpB, kipI, ycsJ
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG2049 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
459,867  460,589
Phenotypes of a mutant
no growth with 5-oxoproline as single source of nitrogen PubMed
reduced growth with ammonium as source of nitrogen due to the accumulation of toxic 5-oxoproline PubMed
The protein
Catalyzed reaction/ biological activity
5-oxoproline + ATP --> glutamate + ADP + Pi PubMed
Protein family
PxpB family (single member, according to UniProt)
Structure
2ZP2 (PDB) PubMed
P60495 (AlphaFold)
Effectors of protein activity
binding of PxpC prevents PxpB from binding to KinA PubMed
Kinetic information
Km (5-oxoproline): 39 M PubMed
Expression and Regulation
Operons
Genes
pxpA-ycsG-ycsI-pxpB-pxpC-kipR-lipC
Description
PubMed
Regulation
induced in the presence of 5-oxoproline PubMed
Regulatory mechanism
KipR: repression, PubMed, in kipR regulon
TnrA: activation, PubMed, in tnrA regulon
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pxpA→lipC

2025-04-03 06:02:48

Jstuelk

102

2e345e8dee0849e85fe094a123ef62dc61c60b82

B227AB4ADF5839F5D85D0A01462013A7CDA6A81D

Biological materials
Mutant
BKE04080 (pxpB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTTTCTCACCTCTTTTA,  downstream forward: _UP4_TATAAGGAGGAGTCCAATTG
BKK04080 (pxpB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTTTCTCACCTCTTTTA,  downstream forward: _UP4_TATAAGGAGGAGTCCAATTG
References
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5A5E17D39296FECC1C03B1A7791AB1065BB4E4DF

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Time of last update: 2025-04-05 23:04:50

Author of last update: Jstuelk