oppA

oppA
168

oligopeptide ABC transporter (binding protein)

Locus
BSU_11430
Molecular weight
61.32 kDa
Isoelectric point
5.72
Protein length
545 aaSequenceBlast
Gene length
1638 bpSequenceBlast
Function
initiation of sporulation, competence development
Product
oligopeptide ABC transporter (binding protein)
Essential
no
Synonyms
oppA, spo0KA
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG4166 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,219,849  1,221,486
Phenotypes of a mutant
reduced survival after DNA replication arrest imposed by inhibition of PolC activity PubMed
The protein
Catalyzed reaction/ biological activity
required for the uptake of quorum sensing peptides such as PhrH PubMed
Protein family
bacterial solute-binding protein 5 family (according to UniProt)
Structure
8ARN (PDB) (complex with SNSS peptide) PubMed
8ARE (PDB) (complex with PhrE peptide) PubMed
1OLA (PDB) (from Salmonella enterica, 36% identity) PubMed
P24141 (AlphaFold)
Modification
phosphorylation on (Tyr-301 OR Tyr-303) AND Thr-470 PubMed
Paralogous protein(s)
DppE
Localization
attached to the cell membrane (via OppB-OppC) PubMed
at the outer side of the cell: extracellular (signal peptide) PubMed,  lipid modification as retention signal PubMed
Expression and Regulation
Operons
Genes
oppA-oppB-oppC-oppD-oppF
Description
PubMed
Regulation
expressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed
Regulatory mechanism
TnrA: activation, PubMed, in tnrA regulon
CodY: repression, no derepression occurs, however, in a CodY mutant, due to increased repression by ScoC PubMed, in codY regulon
ScoC: repression, PubMed, in scoC regulon
Additional information
A ncRNA is predicted between 'trpS' and 'oppA' PubMed
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oppA→oppF

2025-03-19 03:46:36

ghost

135

70070544ac18e9c940a824c6284e4ee6740ee2e5

E173E2B9FD2733A51C073CE2F9C6DE998F2580A2

Biological materials
Mutant
BP67 (spc) available in Fabian Commichau's lab
1S118 ( oppA::spec), PubMed, available at BGSC
GP2097 (D(''oppA-oppB-oppC-oppD-oppF)::aphA3''), available in Jörg Stülke's lab
BKE11430 (''oppA::erm'', available in the BGSC, in Fabian Commichau's, and in Jörg Stülke's lab) PubMed
BKE11430 (oppA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATTCGCAAACCCCCTAA,  downstream forward: _UP4_TAAGGCTACGTCTGAAAATA
BKK11430 (oppA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATTCGCAAACCCCCTAA,  downstream forward: _UP4_TAAGGCTACGTCTGAAAATA
References
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Time of last update: 2025-04-06 04:24:14

Author of last update: Jstuelk