nasE

nasE
168

assimilatory nitrite reductase (subunit)

Locus
BSU_03290
Molecular weight
11.76 kDa
Isoelectric point
4.8
Protein length
106 aaSequenceBlast
Gene length
321 bpSequenceBlast
Function
utilization of nitrite as nitrogen source
Product
assimilatory nitrite reductase (subunit)
Essential
no
E.C.
1.7.1.4
Synonyms
nasE, nasBD, nirD
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG2146 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
355,412  355,732
The protein
Catalyzed reaction/ biological activity
2 H2O + 3 NADP+ + NH4+ --> 5 H+ + 3 NADPH + nitrite  (according to UniProt)
2 H2O + 3 NAD+ + NH4+ --> 5 H+ + 3 NADH + nitrite (according to UniProt)
Domains
Rieske domain (aa 8-104) (according to UniProt)
Cofactors
NAD  PubMed
FeS cluster PubMed
Structure
5BOK (PDB) (from Diaphorobacter sp., 30% identity)
P42436 (AlphaFold)
Localization
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
Genes
nasB-nasC-nasD-nasE-nasF
Description
PubMed
Regulation
''nasD'': expressed under anaerobic conditions (ResD) PubMed
Regulatory mechanism
ResD: activation, PubMed, in resD regulon
TnrA: activation, PubMed, in tnrA regulon
NsrR: repression, PubMed, in nsrR regulon
Fur: repression, PubMed, in fur regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
Open in new tab

nasB→nasF

2025-03-28 12:14:50

ghost

125

f7e39891ce616efe86a59a87e70448f347bb9239

B3FDABF6DE6A38B053CBE30BA36088850B35E312

Genes
nasD-nasE-nasF
Description
PubMed
Regulation
''nasD'': expressed under anaerobic conditions (ResD) PubMed
Open in new tab

nasD→nasF

2025-03-17 09:49:09

ghost

103

5ad87c79ecdfd1b1041c676834950d8393689a20

E10C8C0393696A6DF68E202566914889ADC48A5C

Biological materials
Mutant
BKE03290 (nasE::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTCCATTCCTCCTCAAT,  downstream forward: _UP4_TGATCAAAAGACCGGATCAC
BKK03290 (nasE::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTCCATTCCTCCTCAAT,  downstream forward: _UP4_TGATCAAAAGACCGGATCAC
References
Reviews
Loading
Original Publications
Loading

A8998F46F68155A5C8AE2A2FBB95CA5A1635BB2B

Page visits: 2974

Time of last update: 2025-04-06 13:27:07

Author of last update: Jstuelk