nasC

nasC
168

nitrate reductase (catalytic subunit)

Locus
BSU_03310
Molecular weight
78.39 kDa
Isoelectric point
6.73
Protein length
710 aaSequenceBlast
Gene length
2133 bpSequenceBlast
Function
utilization of nitrate
Product
nitrate reductase (catalytic subunit)
Essential
no
Synonyms
nasC, narB, nasBB
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG3383 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
358,303 → 360,435
The protein
Protein family
prokaryotic molybdopterin-containing oxidoreductase family (according to UniProt)
Domains
4Fe-4S Mo/W bis-MGD-type domain (aa 19-77) (according to UniProt)
Cofactors
MoCo  PubMed
Fe-S cluster  PubMed
Structure
2IV2 (PDB) (from E. coli, 32% identity) PubMed
P42434 (AlphaFold)
Expression and Regulation
Operons
Genes
nasB-nasC-nasD-nasE-nasF
Description
PubMed
Regulation
''nasD'': expressed under anaerobic conditions (ResD) PubMed
Regulatory mechanism
ResD: activation, PubMed, in resD regulon
TnrA: activation, PubMed, in tnrA regulon
NsrR: repression, PubMed, in nsrR regulon
Fur: repression, PubMed, in fur regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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nasB→nasF

2025-03-28 12:14:50

ghost

125

f7e39891ce616efe86a59a87e70448f347bb9239

B3FDABF6DE6A38B053CBE30BA36088850B35E312

Biological materials
Mutant
BKE03310 (ΔnasC::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_AAGCAGTCGTTCAGTCAAAA,  downstream forward: _UP4_TAAATTTTTCATAAAATTTT
BKK03310 (ΔnasC::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_AAGCAGTCGTTCAGTCAAAA,  downstream forward: _UP4_TAAATTTTTCATAAAATTTT
References
Reviews
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Original Publications
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EC8BF9A3BA88FE2A78C6F78A888E0D1E25C47BC5

Page visits: 3553

Time of last update: 2025-04-07 00:11:52

Author of last update: Melvin.boenninger