flhF

flhF
168

signal recognition particle-like GTPase, placement and assembly of flagella, control of basal body position

Locus
BSU_16400
Molecular weight
40.99 kDa
Isoelectric point
8.72
Protein length
366 aaSequenceBlast
Gene length
1101 bpSequenceBlast
Function
placement and assembly of polar flagella
Product
signal recognition particle-like GTPase
Essential
no
Synonyms
flhF
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1419 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,709,747 → 1,710,847
Phenotypes of a mutant
inactivation of ''flhF'' confers resistance to high concentrations of Zn(II) PubMed
defective in Swarming motility PubMed
The protein
Catalyzed reaction/ biological activity
GTPase activity  PubMed
placement of the first flagellar protein, FliF PubMed
Protein family
GTP-binding SRP family (with Ffh and FtsY, according to UniProt)
Structure
2PX3 (PDB) (complexed with GTP-Mg2+) PubMed
3SYN (PDB) (complex with its activator FlhG) PubMed
Q01960 (AlphaFold)
Effectors of protein activity
interaction with FlhG activates the GTPase activity of FlhF PubMed
Localization
cytoplasma PubMed
Expression and Regulation
Operons
Genes
ylxF-fliK-flgD-flgE-swrD-fliL-fliM-fliY-cheY-fliO-fliP-fliQ-fliR-flhB-flhA-flhF-flhG-cheB-cheA-cheW-cheC-cheD-sigD-swrB
Description
PubMed
Regulation
see fla-che operon
Regulatory mechanism
Spo0A: repression, in spo0A regulon
CodY: repression, in codY regulon
DegU: repression, in degU regulon
SwrA: activation, in swrA regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
SigD: sigma factor, PubMed, in sigD regulon
Open in new tab

ylxF→swrB

2025-04-04 21:21:07

Jstuelk

170

EECFE9022742ECC57D9BBA5CE7DB7A4D1BFD42A8

0535E3D13CBD000A94D62050515BF8D1D5E3F011

Genes
flgB-flgC-fliE-fliF-fliG-fliH-fliI-fliJ-ylxF-fliK-flgD-flgE-swrD-fliL-fliM-fliY-cheY-fliO-fliP-fliQ-fliR-flhB-flhA-flhF-flhG-cheB-cheA-cheW-cheC-cheD-sigD-swrB
Description
PubMed
Regulation
expression during spore germination is strongly reduced under conditions of osmotic stress PubMed
Regulatory mechanism
Spo0A: repression, in spo0A regulon
SwrA: activation, in swrA regulon
CodY: repression, in codY regulon
DegU: repression, in degU regulon
SinR: transcription elongation, binding of the SinR-SlrR heteromer to sites within fliE and fliI results in inhibition of transcription elongation PubMed, in sinR regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
SigD: sigma factor, PubMed, in sigD regulon
Open in new tab

flgB→swrB

2025-04-03 01:56:48

Jstuelk

173

024d59ae468aa14568753f57122b789c39ed6c06

4D920B925DA071AE7390E022B9AB625E40B7B2AC

Biological materials
Mutant
1A925 ( flhF::cat), PubMed, available at BGSC
BKE16400 (ΔflhF::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTAAATATCCACCACTCC,  downstream forward: _UP4_CTGTGCAGATGAACAGATAT
BKK16400 (ΔflhF::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTAAATATCCACCACTCC,  downstream forward: _UP4_CTGTGCAGATGAACAGATAT
References
Reviews
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Original Publications
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BB6F5D7463EF96E2C6344D0BC30A227C5E3B5817

Page visits: 5076

Time of last update: 2025-04-06 02:52:06

Author of last update: Jstuelk