yugO

yugO
168

potassium channel protein (exporter)

Locus
BSU_31322
Molecular weight
37.00 kDa
Isoelectric point
7
Protein length
328 aaSequenceBlast
Gene length
987 bpSequenceBlast
Function
potassium exporter
Product
potassium channel protein
Essential
no
Synonyms
yugO
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0569 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,218,854  3,219,840
Phenotypes of a mutant
reduced biofilm formation (can be suppressed by inactivation of ''sinR'') PubMed
The protein
Domains
contains a RCK_N domain (aa 116-245) (according to UniProt)
Structure
3RBZ (PDB) (from Methanothermobacter thermautotrophicus, 24% identity)
Q795M8 (AlphaFold)
Localization
inner spore membrane PubMed
Additional information
potassium exporter activity of YugO is activated by glutamate limitation via the C-terminal domain PubMed
Expression and Regulation
Operons
Genes
mstX-yugO
Description
PubMed
Regulation
repressed by SinR PubMed
Regulatory mechanism
SinR: repression, PubMed, in sinR regulon
Additional information
potassium exporter activity of YugO is activated by glutamate limitation via the C-terminal domain PubMed
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mstX→yugO

2025-04-08 21:46:41

ghost

110

85f2f42956c5da924414be7f576e297b57104a91

B36C37BD378E93291602921CA84AE3502A5DD7AC

Biological materials
Mutant
MGNA-A624 (yugO::erm), available at the NBRP B. subtilis, Japan
GP2098 (yugO::ermC), available in Jörg Stülke's lab PubMed
GP2296 (yugO::tet), available in Jörg Stülke's lab
BKE31322 (yugO::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TGAAATAAATATCCGATTTG,  downstream forward: _UP4_GAAACAGATCAATTCCTTGC
BKK31322 (yugO::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TGAAATAAATATCCGATTTG,  downstream forward: _UP4_GAAACAGATCAATTCCTTGC
FLAG-tag construct
GP2442 yugO-3xFLAG spc (based on pGP1331), available in Jörg Stülke's lab
References
Reviews
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Original Publications
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752860E73AD1A72D6598F995B80F5B21A77A5CBD

Page visits: 5135

Time of last update: 2025-04-07 00:32:22

Author of last update: Jstuelk