khtU

khtU
168

K+/H+ antiporter for K+ efflux

Locus
BSU_09850
Molecular weight
43.79 kDa
Isoelectric point
9.29
Protein length
405 aaSequenceBlast
Gene length
1218 bpSequenceBlast
Function
potassium ion efflux
Product
K+/H+ antiporter for K+ efflux
Essential
no
Synonyms
khtU, yhaU
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0475 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,059,203  1,060,420
Phenotypes of a mutant
increased sensitivity to methylglyoxal PubMed
The protein
Protein family
Monovalent cation:proton antiporter 2 (CPA2) transporter (TC 2.A.37) family (with CpaA, according to UniProt)
Structure
4BWZ (PDB) (sodium proton antiporter NapA from Thermus thermophilus, 26% identity) PubMed
O07536 (AlphaFold)
Effectors of protein activity
potassium export is stimulated by binding of c-di-AMP to KhtT PubMed
Localization
cell membrane, integral membrane protein (according to UniProt)
Expression and Regulation
Operons
Genes
khtS-khtT-khtU
Description
PubMed
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khtS→khtU

2025-04-06 05:35:01

ghost

110

54fb18209326e1c8d64853667143b4fd8e7337e1

ACAE2230F2FD54981F6B24985DF9C660B7B5FFBB

Biological materials
Mutant
MGNA-A682 (yhaU::erm), available at the NBRP B. subtilis, Japan
GP2078 (khtS-khtT-khtU::aphA3), available in Jörg Stülke's lab
BKE09850 (khtU::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_AAATACAAGATGGTCCATCG,  downstream forward: _UP4_GGATAAAAAAAGCAGCACTG
BKK09850 (khtU::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_AAATACAAGATGGTCCATCG,  downstream forward: _UP4_GGATAAAAAAAGCAGCACTG
FLAG-tag construct
GP2432 khtU-3xFLAG spc (based on pGP1331), available in Jörg Stülke's lab
References
Reviews
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Original Publications
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9A54C82323BA9220CAA6041E3745FAB9E222FE78

Page visits: 3533

Time of last update: 2025-04-06 06:28:12

Author of last update: Jstuelk