fliF

fliF
168

flagellar basal-body M-ring protein, membrane anchor of the basal body

Locus
BSU_16210
Molecular weight
59.13 kDa
Isoelectric point
4.37
Protein length
536 aaSequenceBlast
Gene length
1611 bpSequenceBlast
Function
movement and chemotaxis
Product
flagellar basal-body M-ring protein
Essential
no
Synonyms
fliF
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1766 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,692,496 → 1,694,106
Phenotypes of a mutant
mucoid phenotype due to the overproduction of poly-gamma-glutamate PubMed
no secretion of FlgM, permanent inhibition of SigD PubMed
inactivation of ''fliF'' confers resistance to high concentrations of Zn(II) PubMed
not essential for pellicle biofilm formation, but mutant is outcompeted by the wild-type strain when competed during pellicle formation PubMed
defective in Swarming motility PubMed
The protein
Catalyzed reaction/ biological activity
required for correct localization of FliM PubMed
Protein family
FliF family (single member, according to UniProt)
Structure
6SCN (PDB) (from Salmonella typhimurium, corresponds to aa 16 ... 375, 27% identity)
P23447 (AlphaFold)
Localization
cell membrane PubMed
Expression and Regulation
Operons
Genes
flgB-flgC-fliE-fliF-fliG-fliH-fliI-fliJ-ylxF-fliK-flgD-flgE-swrD-fliL-fliM-fliY-cheY-fliO-fliP-fliQ-fliR-flhB-flhA-flhF-flhG-cheB-cheA-cheW-cheC-cheD-sigD-swrB
Description
PubMed
Regulation
expression during spore germination is strongly reduced under conditions of osmotic stress PubMed
Regulatory mechanism
Spo0A: repression, in spo0A regulon
SwrA: activation, in swrA regulon
CodY: repression, in codY regulon
DegU: repression, in degU regulon
SinR: transcription elongation, binding of the SinR-SlrR heteromer to sites within fliE and fliI results in inhibition of transcription elongation PubMed, in sinR regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
SigD: sigma factor, PubMed, in sigD regulon
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flgB→swrB

2025-04-03 01:56:48

Jstuelk

173

024d59ae468aa14568753f57122b789c39ed6c06

4D920B925DA071AE7390E022B9AB625E40B7B2AC

Biological materials
Mutant
DS7080 (marker-less  in NCIB3610) PubMed
BKE16210 (ΔfliF::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTTAACCCCCGGTCATT,  downstream forward: _UP4_GCCGAGGATTAGGAGGAATT
BKK16210 (ΔfliF::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTTAACCCCCGGTCATT,  downstream forward: _UP4_GCCGAGGATTAGGAGGAATT
References
Reviews
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Original Publications
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EB815705133E3318F655F6024B7D9BA587FD1DDA

Page visits: 4725

Time of last update: 2025-04-10 13:45:53

Author of last update: Jstuelk