spxH

spxH
168

adaptor protein for ClpX-ClpP-catalyzed Spx degradation, confers resistance against nitrosating agents

Locus
BSU_11550
Molecular weight
31.33 kDa
Isoelectric point
5.21
Protein length
299 aaSequenceBlast
Gene length
900 bpSequenceBlast
Function
stimulation of Spx degradation
Product
adaptor protein
Essential
no
Synonyms
yjbH
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG2761 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,233,614  1,234,513
Phenotypes of a mutant
increased thermotolerance due to increased stabiliy of Spx and thus increased expression of ''trxA'' PubMed
defective in Swarming motility (periodic cessation and reinitiation of Swarming motility, terracing colonies) PubMed
The protein
Catalyzed reaction/ biological activity
adaptor protein for ClpX-ClpP-catalyzed Spx degradation PubMed
Protein family
UPF0413 family (single member, according to UniProt)
Cofactors
contains Zn atoms (coordinated by the N-terminal His-rich region) PubMed
Structure
6GHB (PDB) (oxidized Geobacillus kaustophilus protein complexed with B. subtilis Spx) PubMed
6GHO (PDB) (Geobacillus kaustophilus protein complexed with B. subtilis Spx) PubMed
O31606 (AlphaFold)
Effectors of protein activity
SpxH aggregates under stress conditions, resulting in the inability to bind to Spx, and therefore in stabilization of Spx PubMed
Zn atom is released upon treatment with strong oxidants PubMed
interaction with YirB inhibits the formation of a complex with Spx PubMed
Localization
cytosolic protein
aggregates localize at nucleoid-free regions of the cell PubMed
Expression and Regulation
Operons
Genes
yjbI-spxH
Description
PubMed
Regulation
induced by cell wall stress (Spx) PubMed
Regulatory mechanism
Spx: activation, PubMed, in spx regulon
Additional information
A ncRNA is predicted between yizD and spxH PubMed
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yjbI→spxH

2025-03-18 12:49:47

Jstuelk

87

8d759f27656c90574831e1b626e779b9f73bc246

DDE7E06D5F7C0635D74C904968069D697AA98842

Biological materials
Mutant
GP2645 (spxH::erm), available in Jörg Stülke's lab
BKE11550 (''spxH::erm'', available in the BGSC and in Jörg Stülke's lab) PubMed
BKE11550 (spxH::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GTATAGCTCATGCTGATAGT,  downstream forward: _UP4_TAGCCGCAGGCGTGCATATG
BKK11550 (spxH::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GTATAGCTCATGCTGATAGT,  downstream forward: _UP4_TAGCCGCAGGCGTGCATATG
Two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
FLAG-tag construct
GP3799 (spc, based on pGP1331), available in Jörg Stülke's lab
Labs working on this gene/protein
Peter Zuber, Oregon Health and Science University, USA
Homepage
Claes von Wachenfeldt, Lund University, Sweden Homepage
References
Reviews
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Original Publications
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87124945A1CBF7990856FBEB9EAD25096DAC0868

Page visits: 5532

Time of last update: 2025-04-06 19:23:25

Author of last update: Dennis.wicke