yabT

yabT
168

Ser/Thr kinase, controls Translation and DNA integrity during spore development

Locus
BSU_00660
Molecular weight
37.51 kDa
Isoelectric point
9.94
Protein length
338 aaSequenceBlast
Gene length
1017 bpSequenceBlast
Function
control of DNA integrity during spore development
Product
Ser/Thr kinase
Essential
no
Synonyms
yabT
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0515 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
73,809 → 74,825
Phenotypes of a mutant
the mutant tends to acquire suppressor mutations that result in improved growth PubMed
increased sensitivity to DNA damage during spore development PubMed
The protein
Catalyzed reaction/ biological activity
phosphorylation of RecA on Ser-2 PubMed
phosphorylation of EF-Tu on Thr-63 during sporulation PubMed
phosphorylation of YabA on Thr-71 PubMed
ATP + L-seryl-[protein] --> ADP + H+ + O-phospho-L-seryl-[protein] (according to UniProt)
ATP + L-threonyl-[protein] --> ADP + H+ + O-phospho-L-threonyl-[protein] (according to UniProt)
Protein family
protein kinase superfamily (according to UniProt)
Ser/Thr protein kinase family (according to UniProt)
Domains
Protein kinase domain (aa 28-286) (according to UniProt)
Structure
6G4J (PDB) PubMed
P37562 (AlphaFold)
Effectors of protein activity
autophosphorylation is stimulated by non-specific binding to DNA PubMed
Localization
colocalizes strongly with the septal membrane separating the mother cells from the forespore PubMed
Expression and Regulation
Operons
Genes
spoIIE-yabS-yabT-tilS-hprT-ftsH
Description
PubMed
Regulation
expressed early during sporulation in the forespore (SigF) PubMed
Sigma factors
SigF: sigma factor, PubMed, in sigF regulon
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spoIIE->ftsH

2025-04-03 08:25:04

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Biological materials
Mutant
MGNA-B921 (yabT::erm), available at the NBRP B. subtilis, Japan
GP577 (erm), available in Jörg Stülke's lab
BKE00660 (ΔyabT::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTTGATTGTCGTACCCGGCT,  downstream forward: _UP4_TGAATGGTGCAAACTGCAGA
BKK00660 (ΔyabT::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTTGATTGTCGTACCCGGCT,  downstream forward: _UP4_TGAATGGTGCAAACTGCAGA
Expression vectors
purification from B. subtilis with N-terminal Strep-tag, for SPINE, in pGP380: pGP391, available in Jörg Stülke's lab
purification from E. coli with N-terminal Strep-tag, in pGP172: pGP823, available in Jörg Stülke's lab, PubMed
purification from E. coli with N-terminal His-tag, in pWH844: pGP1408, available in Jörg Stülke's lab
LacZ fusion
translational lacZ fusion (in pAC7): pGP831, available in Jörg Stülke's lab
References
Reviews
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Original Publications
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E86A96B832351FF513DD9853EAD8998CC44C9951

Page visits: 4586

Time of last update: 2025-04-06 07:52:49

Author of last update: Christoph.elfmann