ydaP
168

pyruvate:quinone oxidoreductases, general stress protein, required for survival of ethanol stress, cold stress (4°C) and oxidative stress (superoxide/paraquate)

Locus

BSU_04340

Molecular weight

62.97 kDa

Isoelectric point

5.22

Protein length

574 aaSequenceBlast

Gene length

1725 bpSequenceBlast

Function

utilization of pyruvate

Product

pyruvate:quinone oxidoreductases

Essential

Synonyms

ydaP

Outlinks

BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Hide small RNAsZoom:

Categories containing this gene/protein

Metabolism, Carbon metabolism, Utilization of specific carbon sources, Utilization of organic acids

Lifestyles, Coping with stress, General stress proteins (controlled by SigB)

Groups of genes, Membrane proteins

Groups of genes, Poorly characterized/ putative enzymes

List of homologs in different organisms, belongs to COG0028 (Galperin et al., 2021)

This gene is a member of the following regulons

SigB regulon

Gene

Coordinates

488,830 490,554

Phenotypes of a mutant

sensitive to ethanol, cold (4C) and superoxide stress (paraquat)

The protein

Catalyzed reaction/ biological activity

pyruvate + menaquinone --> acetate + menaquinol + CO₂ PubMed

Protein family

TPP enzyme family (according to UniProt)

Cofactors

FAD PubMed

TPP PubMed

Mg²⁺ PubMed

Structure

4KGD (PDB) (the enzyme from Lactobacillus plantarum, 37% identity, 70% similarity) PubMed

1POW (PDB) (from Lactobacillus plantarum, 38% identity)

P96591 (AlphaFold)

Paralogous protein(s)

AlsS, IlvB

Localization

peripheral membrane proteins bound to the cytoplasmatic side of the membrane PubMed

Expression and Regulation

Operons

Genes

ydaP

Description

PubMed

Regulation

induced by stress (SigB) PubMed

Sigma factors

SigB: sigma factor, PubMed, in sigB regulon

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Biological materials

Mutant

MGNA-C115 (ydaP::erm), available at the NBRP B. subtilis, Japan

GP457 (spc), available in Jörg Stülke's lab

JH642 and its derivatives: natural deletion of the 18 kb ydzA-mntH region encompassing the genes ydzA-topB-ydaJ-ydaK-ydaL-ydaM-ydaN-kimA-mutT-ydaP-ydzK-mntH PubMed

BKE04340 (ydaP::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAACGTATCCTCCTTTTT, downstream forward: _UP4_TAAAAAAACAGGGGCCCTAA

BKK04340 (ydaP::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAACGTATCCTCCTTTTT, downstream forward: _UP4_TAAAAAAACAGGGGCCCTAA

References

Reviews

Original Publications

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Time of last update: 2025-04-06 17:10:51

Author of last update: Jstuelk

ydaP 168

ydaP
168