pftB

pftB
168

pyruvate transporter

Locus
BSU_28900
Molecular weight
24.25 kDa
Isoelectric point
9.81
Protein length
231 aaSequenceBlast
Gene length
696 bpSequenceBlast
Function
uptake of pyruvate
Product
pyruvate transporter
Essential
no
Synonyms
pftB, ysbB
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1346 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,954,492 → 2,955,187
Phenotypes of a mutant
strongly impaired growth with pyruvate as the single carbon source PubMed
a ''ywbH-ywbG yxaK-yxaC pftA-pftB'' triple mutant is severely delayed in pellicle formation PubMed
The protein
Catalyzed reaction/ biological activity
uptake of pyruvate PubMed
Protein family
CidB/LrgB family (single member, according to UniProt)
Structure
P94516 (AlphaFold)
Localization
cell membrane PubMed
Expression and Regulation
Operons
Genes
pftA-pftB
Description
PubMed
Regulation
induced by acetate PubMed
induced by pyruvate (LytT) PubMed
subject to carbon catabolite repression (CcpA) PubMed
Regulatory mechanism
LytT: activation, PubMed, in lytT regulon
CcpA: repression, (PubMed, in ccpA regulon
Kre: repression, in kre regulon
Additional information
the pftA-pftB operon is strongly upregulated in a ''kre'' mutant PubMed
the pftA-pftB operon is strongly upregulated in a ''cshA'' mutant PubMed
Open in new tab

pftA→pftB

2025-03-10 23:21:24

Jstuelk

104

ce4916b0164540e2bd6942d8bdd1a80a4d3d3248

0250E8970D3DB5ED35BF13FB7FD8459929EA2708

Biological materials
Mutant
MGNA-A983 (ysbB::erm), available at the NBRP B. subtilis, Japan
GP1554 (''pftA-pftB::spc''), available in Jörg Stülke's lab
BKE28900 (ΔpftB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTGAAGAACCTCCTGTT,  downstream forward: _UP4_TAAGCCAAGGCTGAATGCCT
BKK28900 (ΔpftB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTGAAGAACCTCCTGTT,  downstream forward: _UP4_TAAGCCAAGGCTGAATGCCT
GP2577 (ΔpftB::tet comIQ12L) (in DK1042) available at Jörg Stülke's lab
References
Reviews
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Original Publications
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F6C3B267106E7510FABECF0B13ABF222901689A9

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Time of last update: 2025-04-02 07:51:34

Author of last update: Jstuelk