clpX

clpX
168

AAA unfoldase, ATP-dependent Clp protease, ATP-binding subunit (class III heat-shock protein)

Locus
BSU_28220
Molecular weight
46.25 kDa
Isoelectric point
4.64
Protein length
420 aaSequenceBlast
Gene length
1263 bpSequenceBlast
Function
protein degradation
Product
AAA unfoldase, ATP-dependent Clp protease, ATP-binding subunit
Essential
no
Synonyms
clpX
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1219 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,884,781  2,886,043
Phenotypes of a mutant
increased thermotolerance due to increased stabiliy of Spx and thus increased expression of ''trxA'' PubMed
the mutation suppresses the heat sensitivity of spores overexpressing ''cmpA'' PubMed
defective în sporulation PubMed
defective in Swarming motility PubMed
The protein
Catalyzed reaction/ biological activity
ATPase/chaperone
Protein family
ClpX chaperone family (with ClpY, according to UniProt)
Domains
AAA-ATPase PFAM
Zinc finger PFAM
Structure
1UM8 (PDB) (from Helicobacter pylori) PubMed
P50866 (AlphaFold)
Localization
cytoplasmic polar clusters, excluded from the nucleoid, induced clustering upon heat shock, colocalization with ClpP PubMed
Expression and Regulation
Operons
Genes
clpX
Description
PubMed
Regulation
induced by heat stress (CtsR) PubMed
Regulatory mechanism
CtsR: repression, PubMed, in ctsR regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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clpX

2025-04-04 19:25:57

ghost

97

5e2b40cda08a3510c2ef35902046c624ab3fe2d8

D2F86E6B696247121B02A464AD0553FF86B67526

Biological materials
Mutant
MGNA-B019 (clpX::erm), available at the NBRP B. subtilis, Japan
GPUG2 (aphA3), available in Ulf Gerth's and Jörg Stülke's labs
GP1787 (aphA3), available in Jörg Stülke's lab
clpX::kan, clpX::spec and clpX::cat available from the Hamoen] Lab
BKE28220 (clpX::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTCTTTCACCCCTTAATC,  downstream forward: _UP4_TAAAGATAAGCACAAACCTC
BKK28220 (clpX::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTCTTTCACCCCTTAATC,  downstream forward: _UP4_TAAAGATAAGCACAAACCTC
Expression vectors
pGP3700 (N-terminal 6xHis-tag, purification from E. coli, in pWH844), available in Jörg Stülke's lab
GFP fusion
C-terminal GFP fusions (both single copy and 2th copy in amyE locus, also as CFP and YFP variants) available from the Hamoen] Lab
Labs working on this gene/protein
Leendert Hamoen, Newcastle University, UK homepage
References
Reviews
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Original Publications
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Page visits: 8187

Time of last update: 2025-04-10 06:38:58

Author of last update: Jstuelk